不同冲洗剂冲洗根管杀菌效果的体外观察  

Microbicidal Efficacy Observation in vitro between Sodium Hypochlorite and Ozonated Water of Different Concentration

作　　者：高爽[1,2] 李朋莲[1,2] 李振玲[1,2] 刘佳柏[1,2] 卢春英[1,2] 张志民[1,2] 

机构地区：[1]吉林大学口腔医院 [2]吉林大学口腔医学院,吉林长春130021

出　　处：《吉林医学》2014年第10期2030-2032,共3页Jilin Medical Journal

基　　金：吉林省科技厅基金资助项目[项目编号:3D1062493431]

摘　　要：目的:比较次氯酸钠溶液与不同浓度臭氧水溶液的杀菌效果,为臭氧水溶液应用于临床感染根管的冲洗提供微生物学基础。方法:将根尖发育完全的单直根管离体牙经一系列标准化处理后,建立离体牙的E.faecalis体外感染模型,采用Mtwo机用镍钛器械和冠向下法预备根管,稀释平板计数法分别测定各实验组冲洗前、冲洗后24 h和7 d时E.faecalis的CFUml-1。Mann-Whitney U检验法进行数据统计分析。结果:冲洗前各组根管内细菌数量的差异无统计学意义(P>0.05)。经冲洗剂处理后,培养24 h各实验组对根管内E.faecalis的杀灭率均为100%,7 d时均有少量E.faecalis被检出,但三组之间无统计学差异(P>0.05)。结论:5.25%NaCl溶液、3.994 mg/L臭氧水溶液和1.339 mg/L臭氧水溶液对根管内的E.faecalis均有瞬时杀灭作用,但却不能完全将其清除,只能减少它的数量,使其在一段时间内处于抑制状态;臭氧水溶液对根管内E.faecalis的杀灭能力与其浓度呈正相关。Objective To evaluate the microbicidal efficacy between the sodium hypochlorite and the ozonated water of different concentration, provide the microbiological foundation for the application of ozonated water as a root canal irrigating agent in clinical. Method Standardize the extracted human tooth. After establishing the E. faeealis infected model, preparing the roots through the step - down technique with sterile Mtwo machine nickel - titanium instruments. Using the plate count assay the CFU/ml of E. faecalis before and after irrigation. Data was analyzed by Mann - Whitney tests. Results Before irrigation, there was no statistic differences of the CFU among three groups （ P 〉 0. 05）. After irrigation, the 24 - hour killing ratio of the three irrigating agents on E. faecalis in root canals were all 100% . But after 7 days, a small amount of E. faecalis had been detected ,The difference was not statistically significant among the three groups （ P 〉 0.05 ）. Conclusion The E. faecalis in root canals can be killed instantaneously by the three irrigating agents,but can not be removed off,can only reduce in its quantity and inhibited during a period of time;the concentration and the microbicidal efficacy of ozonated water are positively correlated.

关 键 词：臭氧水溶液 粪肠球菌 杀菌能力 

分 类 号：R781.05[医药卫生—口腔医学]