南瓜多糖含量测定方法比较  

The Content of Pumpkin Polysaccharide Determination Comparison

作　　者：陈立江[1] 王菁[1] 刘宇[1] 焉喜臣[1] 王金晶[1] 吴凡[1] 

机构地区：[1]辽宁大学药学院,辽宁沈阳110036

出　　处：《辽宁大学学报（自然科学版）》2014年第1期76-81,共6页Journal of Liaoning University：Natural Sciences Edition

摘　　要：确立一种能够准确测定南瓜多糖含量的方法.采用间接碘量法和苯酚硫酸法分别测定同一批样品南瓜多糖的含量.实验结果表明,间接碘量法测定南瓜多糖的含量平均为43.61%,其中总糖为53.14%,单糖为9.53%.总糖和单糖的平均回收率分别为100.09%和101.51%,相对标准偏差(RSD)为0.63%和0.77%,重现性良好.间接碘量法不仅能测出总糖含量,并且也能对单糖含量有较好评价.该方法准确,简便,稳定性好.A method was developed to determine the contents of pumpkin Polysaccharides accurately. The contents of Pumpkin Polysaccharides in the same batch was determined by indirect iodometric method and Phenol-sulfuric Acid. Experimental results of indirect iodometfic method show that the average content of Pumpkin Polysaccharides is 43.61%, as the total sugar content is 53.14% and the monosaccharide is 9.53%. The average recovery of total sugar and monosaccharide is 100.09% and 101.51% respectively,and the relative standard deviation（RSD） is 0.63% and 0.77% respectively, with good reproducibility. Indirect iodometric method can evaluate the determination of Pumpkin Polysaccharides on total sugar and monosaccharide. This method is accurate, simple and steady.

关 键 词：南瓜多糖 间接碘量法 苯酚硫酸法 含量测定 

分 类 号：O629.1[理学—有机化学]