紫外线辐照对纯钛喷砂酸蚀表面理化性能及人纤连蛋白吸附的影响  

The influence of ultraviolet irradiation on sandblasted and acid-etching surface adsorbing human fibronectin

作　　者：李少冰[1] 张雪洋[1] 倪佳[2] 韦从云[3] 容明灯[1] 周磊[4] 

机构地区：[1]广东省口腔医院·南方医科大学附属口腔医院牙周种植科,广州510280 [2]广东省口腔医院·南方医科大学附属口腔医院牙周科,广州510280 [3]深圳牙科医疗中心 [4]广东省口腔医院·南方医科大学附属口腔医院种植中心,广州510280

出　　处：《中华口腔医学杂志》2014年第4期234-238,共5页Chinese Journal of Stomatology

基　　金：广东省自然科学基金（S2013040014986）;广东省医学科学技术研究基金（A2013121）

摘　　要：目的 研究紫外线辐照对纯钛喷砂酸蚀表面理化性能及吸附人纤连蛋白（human fibronectin,HFn）的影响,为评估紫外线-喷砂酸蚀纯钛表面的生物学活性提供参考.方法 分别制备喷砂酸蚀表面和紫外线-喷砂酸蚀表面的纯钛试件（每组60个）,比较两组表面形貌、粗糙度、元素组成、润湿性及HFn吸附的差异.结果 两组有相似的多级孔洞及平均粗糙度.紫外线-喷砂酸蚀组纯钛试件表面有较低的C（22.83％）和较高的O（51.20％）并呈现亲水性;而喷砂酸蚀组呈现疏水性,其吸附10 min时HFn吸附量[（0.41±0.07）μg]显著高于紫外线-喷砂酸蚀组[（0.26±0.08）μg]（P =0.007）.结论 紫外线辐照并未改变纯钛喷砂酸蚀表面的形貌结构及粗糙度,但可降低表面碳氢化合物并增加羟基,吸附蛋白10 min时紫外线-喷砂酸蚀钛表面的HFn体外吸附量显著低于喷砂酸蚀钛表面.因此,紫外线-喷砂酸蚀钛表面的体外生物学活性尚未及喷砂酸蚀钛表面.Objective To study the influence of ultraviolet （UV） irradiation on physical and chemical properties of sandblasted and acid-etched （SA） titanium surface and their ability to adsorb human fibronectin （HFn）.Methods SA and UV-SA surfaces were separately prepared.Surface morphology,roughness,elemental composition,wettability and HFn adsorption assay were performed for comparative analysis of these two surfaces.Results SA and UV-SA surface had a similar morphology with multi-holes,and average roughness.UV-SA surface had a lower C content （22.83％） and higher O content （51.20％） and presented hydrophilicity,while SA surface showed hydrophobicity.But the quantity of adsorbed HFn on SA surface at 10 min assay point [（0.41 ±0.07） μg] was statistically higher than that on UV-SA surface [（0.26 ± 0.08） μg] （P =0.007）.Conclusions UV irradiation would not change the morphology and roughness of SA surface.However,it could reduce the hydrocarbon and increase the hydroxyl groups,and the absorption of HFn on UV-SA surface at 10 min assay point was statistically lower than that on SA surface.Therefore,the in-vitro bioactivity of UV-SA surface was not as good as that of SA surface.

关 键 词：钛 紫外线 化学 物理 纤连蛋白类 吸附 

分 类 号：R783.6[医药卫生—口腔医学]