镍钛-不锈钢激光焊复合弓丝细胞毒性的检测  

Cytotoxicitic detection of laser welding between NiTi shape memory alloy and stainless steel in vitro

作　　者：于文雯 张超[1] 张兵[1] 刘继明[1] 孙新华[1] 

机构地区：[1]吉林大学口腔医学院正畸科,长春130021

出　　处：《中华口腔医学杂志》2014年第4期239-243,共5页Chinese Journal of Stomatology

摘　　要：目的 检测镍钛-不锈钢激光焊复合弓丝的细胞毒性,并与不锈钢弓丝、镍钛弓丝比较,探讨复合弓丝的生物安全性.方法 将镍钛-不锈钢复合弓丝浸提液配置成体积分数为50％、40％、30％、20％、10％的稀释液,采用甲基噻唑基四唑（methyl thiazolyl tetrazolium,MTT）检测培养24、48 h后各稀释液对小鼠成纤维细胞L929的细胞毒性.将复合弓丝、不锈钢弓丝、镍钛弓丝浸提液配置成体积分数为100％、75％、50％、25％的稀释液,采用MTT检测并比较48 h后各稀释液对L929的细胞毒性.结果 各浸提液细胞毒性均为0～1级.除20％复合弓丝浸提液外,相同体积分数复合弓丝浸提液与L929共培养48 h后A值均显著低于共培养24 h.100％、75％、50％、25％复合弓丝浸提液A值（1.964±0.122、2.084±0.056、2.056±0.071、2.096±0.050）均显著低于不锈钢弓丝浸提液（2.168 ±0.091、2.227±0.160、2.302±0.052、2.301±0.060）、镍钛弓丝浸提液（2.138±0.105、2.262±0.050、2.271 ±0.082、2.294 ±0.056） （P ＜0.05）.结论 复合弓丝的细胞毒性作用与浸提液体积分数、培养时间有一定相关性,较不锈钢弓丝、镍钛弓丝明显.但复合弓丝的细胞毒性作用较小,在材料毒性反应安全级别范围内.Objective To investigate the cytotoxicity of laser-welded nickel titanium （NiTi） and stainless steel composite archwire.Methods The NiTi and stainless steel composite archwire （CoAW） laser-welded with pure copper inrerplayer was studied with methyl thiazolyl tetrazolium （MTT） test in vitro.The cytotoxicity of CoAW was compared with stainless steel archwire and NiTi archwire.Two tests were carried out.Test 1：the immersed solution of CoAW was diluted to five grades （50％,40％,30％,20％,10％）.The cytotoxicity in vitro of these agents was assayed on murine fibroblast cell L929 line with MTT test at 24 and 48 hours.Test 2：the immeresed solution of CoAW,NiTi archwires and stainless steel archwires was diluted to four grads （100％,75％,50％,25％）.The cytotoxity of three kinds of material was compared at 48 hours.Results The results of all samples revealed level 0-1 cytotoxicity.In test 1,the same grade solution optical density （except 20％） at 24 hours was statistically lower than at 48 hours.In test 2,the optical density of CoAW solution （1.964 ± 0.122,2.084 ± 0.056,2.056 ± 0.071,2.096 ± 0.050） was statistically lower than the same grade solution of stainless steel archwire （2.168 ± 0.091,2.227 ± 0.160,2.302 ± 0.052,2.301 ± 0.060） and NiTi archwire （2.138 ± 0.105,2.262 ± 0.050,2.271 ± 0.082,2.294 ± 0.056） （P ＜ 0.05）.Conclusions The MTT test of CoAW in vitro showed that cytotoxicity was related to concentration and time.The cytotoxicity of the CoAW was more serious than that of stainless steel and NiTi archwires.However,CoAW belonged to secure rang of material toxicity reaction.

关 键 词：正畸金属丝 焊接 细胞毒性 免疫 

分 类 号：R783.5[医药卫生—口腔医学]