健康青年正常牙本质中基质金属蛋白酶2的定量检测初探  

Quantitative detection of matrix metalloproteinase-2 in normal coronal dentine of young people

作　　者：王丹杨[1] 张凌[1] 李芳[1] 陈宇江[2] 陈吉华[1] 

机构地区：[1]第四军医大学口腔医学院修复科,西安710032 [2]第四军医大学口腔医学院儿童口腔科,西安710032

出　　处：《中华口腔医学杂志》2014年第4期244-246,共3页Chinese Journal of Stomatology

基　　金：国家自然科学基金（51373198、81100772、81130078）

摘　　要：目的 定量检测青年人群正常牙本质中基质金属蛋白酶2（matrix metalloproteinase-2,MMP-2）的含量及分布情况.方法 选择离体前磨牙30颗和第三磨牙27颗（患者年龄20 ～ 30岁）,纵切成1.5 mm厚的牙本质片,磨除釉质后将冠部牙本质分成浅层和深层两部分,分别制备成牙本质粉,脱矿、提取蛋白后利用液相芯片技术定量检测MMP-2含量.结果 青年人群前磨牙牙本质浅层和深层MMP-2分别为（0.022±0.006）和（2.087±0.090） ng/mg,磨牙牙本质浅层和深层MMP-2含量分别为（0.336±0.037）和（3.312±0.308） ng/mg;各牙位深层牙本质MMP-2含量均显著高于浅层,磨牙同层次牙本质MMP-2含量均显著高于前磨牙（P＜0.05）.结论 健康青年正常牙本质中含MMP-2,不同牙位及牙本质层次MMP-2含量不同.Objective To compare the distribution and concentration of matrix metalloproteinases-2 （MMP-2） in different dentin depth of premolar and molar of young people.Methods Freshly extracted human premolars and molars （aged between 20-30） were sectioned to 1.5 mm thick slices along the longitudinal axis of the tooth separately.Enamel and pulp of each slice was removed,and then the premolar and molar slices were respectively divided into two subgroups according to superficial or deep dentin and pulverized to fine powder.After dentin protein was extracted,the concentrations of MMP-2 in different tooth were detected using fluorescent microsphere immunoassay.Results The content of MMP-2 in superficial layer dentin of premolar was （0.022 ± 0.006） ng/mg.The content of MMP-2 in deep layer dentin of premolar was （2.087 ± 0.090） ng/mg.The content of MMP-2 in superficial layer dentin of molar was （0.336 ± 0.037） ng/mg.The content of MMP-2 in deep layer dentin of molar was （3.312 ± 0.308） ng/mg.Conclusions MMP-2 exists in human coronal dentin.In the same type of teeth of young people,the concentration of MMP-2 in deep dentin was significant higher than those in superficial dentin.In the same dentin depth,the concentration of MMP-2 in molar was significant higher than those in premolar.

关 键 词：牙本质 基质金属蛋白酶2 液相芯片技术 

分 类 号：R781[医药卫生—口腔医学]