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作 者:张松[1,2] 田向永 袁孝伟[3] 柯少瑞[3] 孙志艳[4] 莫赛军[3]
机构地区:[1]郑州大学第一附属医院,郑州市450052 [2]郑州人民医院放疗科 [3]郑州大学基础医学院基础肿瘤学教研室 [4]中国人民解放军第一五三中心医院全军创伤骨科中心
出 处:《中国肿瘤临床》2014年第7期449-452,共4页Chinese Journal of Clinical Oncology
基 金:国家自然科学基金项目(编号:81101686);河南省教育厅科学技术研究重点基金项目(编号:12A310008)资助~~
摘 要:目的:探讨窖蛋白-1(Caveolin-1,Cav-1)在腮腺多形性腺瘤(pleomorphic adenoma,PA)中的表达及临床意义。方法:采用免疫组织化学法检测了9例正常腮腺、33例原发性PA距离瘤体中心(0 cm)及瘤旁组织中(分别距瘤体中心0.5、1.0、1.5和2.0 cm)Cav-1的表达,并进行统计学分析。结果:Cav-1在所有正常腮腺样本(100%)均有表达,其主要在导管系统细胞质和细胞膜中表达;除6例距瘤体中心0.5 cm和1例距瘤体中心1.0 cm的样本外,其余瘤旁样本均有Cav-1表达,其表达模式基本上与正常腮腺类似;在PA中,有10例样本(30.30%)表达Cav-1,且在细胞核中显著表达。Cav-1在多形性腺瘤肿瘤组织中的表达最低,并随着与瘤体中心距离的远近,其表达量逐渐升高。Cav-1的表达在瘤体与瘤旁组织间均存在明显差异(P<0.05),但瘤旁组织1.0、1.5和2.0 cm间Cav-1表达无显著性差异(P>0.05)。结论:在腮腺多形性腺瘤发生过程中,Cav-1中表达下调并出现了从细胞质、细胞膜到细胞核的转位现象。上述结果表明Cav-1在多形性腺瘤的发生发展中可能起着重要作用,其表达水平的变化可作为判定手术安全边缘的科学依据之一。Objective:This study was conducted to explore the expression and clinical significance of caveolin-1 (Cav-1) in pleo-morphic adenoma and paraneoplastic tissues of the parotid gland. Methods:Immunohistochemistry was used to detect Cav-1 expres-sion in normal parotid glands, pleomorphic adenoma (0 cm), and paraneoplastic tissues (0.5, 1.0, 1.5, and 2.0 cm from the pleomorphic adenoma). Results: Cav-1 was expressed in all normal parotid glands and was mainly expressed in the cytoplasm and plasma mem-brane of duct systems. Except for six samples 0.5 cm away and one sample 1.0 cm away from the pleomorphic adenoma, Cav-1 was ex-pressed in other adjacent normal tissues, and the expression pattern of Cav-1 was similar to that in normal tissues. In pleomorphic ade-noma, Cav-1 only existed in seven samples (accounting for 30.30%) and was prominently expressed in the nucleus. The expression lev-el of Cav-1 was lowest in pleomorphic adenoma and progressively increased with increasing distance from the tumor. A difference in Cav-1 expression was detected between pleomorphic adenoma (0 cm) and paraneoplastic tissues (0.5, 1.0, 1.5, and 2.0 cm from the tu-mor, P〈0.05). However, no statistical significance existed between paraneoplastic tissues at 1.0, 1.5, and 2.0 cm from the tumor (P〉0.05). Conclusion:During the development of pleomorphic adenoma, the expression level of Cav-1 progressively decreased, and trans-location of Cav-1 expression from cytoplasm and plasma membrane to nucleus occurred. Cav-1 may have an important role in the prog-ress of pleomorphic adenoma, and the variation in the expression level of Cav-1 may be used as a scientific evidence to determine the safe surgical margin of pleomorphic adenoma.
关 键 词:CAVEOLIN-1 多形性腺瘤 腮腺 手术切缘
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