CXCR4在低氧诱导的上颌窦癌细胞IMC3中的表达及其意义  

Up-regulation of the CXCR_4 expression by hypoxia in maxillary sinus carcinoma cells IMC_3

作　　者：苏立众[1,2] 张志明[3] 季文樾[1] 

机构地区：[1]中国医科大学附属盛京医院耳鼻咽喉科,沈阳110004 [2]浙江省人民医院耳鼻咽喉科,杭州310014 [3]沈阳解放军第202医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第8期548-550,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:研究CXCR4在低氧诱导的上颌窦癌细胞IMC3中的表达及其意义。方法:Real-Time PCR测定低氧6h、12h、24h、48hIMC3中CXCR4的mRNA表达量,并行免疫组织化学研究。结果:CXCR4mRNA正常状态下为0.035,低氧6h、12h、24h、48h分别为0.283、0.313、0.426、0.510。除低氧6h和12h组相比差异无统计学意义外,其余各组间差异有统计学意义(P<0.05)。免疫组织化学结果表明,低氧下CXCR4的mRNA表达主要在细胞膜和细胞质,少量在细胞核。结论:低氧能诱导IMC3细胞CXCR4mRNA的表达,随低氧时间延长而增高,有明显时间性。Objective：To investigate the expression of CXCR4 in maxillary sinus carcinoma cells- IMC3 under hypoxia. Nlethod：IMC3 cells were cultured for 6 h, 22 h, 24 h and 48 h under normoxia and hypoxia. Real-Time PCR was applied to detect the expression of mRNA of CXCR4 and immunohistochemisrty was applied to investi- gate its protein level. Result：CXCR4 mRNA level was about 0. 035 under normal conditions, which was obviously upregulated by hypoxia. The mRNA levels after culturing under hypoxia for 6 h,12 h,24 h and 48 h were 0. 283, 0. 313, 0. 426, 0. 510 respectively. There was statistically significant difference between the mRNA levels of each two groups（P（0.05, Mann-Whiney Test）with a time dependent course, except for the difference between the groups of 6 h and 12 h. Immunohistochemistry showed that there was almost negative staining for CXCR4 in the cell cultured in nomoxia, while stong positive staining of CXCR4 was observed in cells cultured in hypoxia . The positive staining was located mainly in the cell membrane and cytoplasm and little in the nucleus. Conclusion： Hy- poxia could induce expression of CXCR4 in IMC3 cells at both mRNA and ptrotein levels. The upregulation of CX- CR4 by hypoxia showed an obvious time dependent course.

关 键 词：趋化因子受体CXCR4 上颌窦肿瘤 实时定量PCR 

分 类 号：R739.62[医药卫生—肿瘤]