CacyBP/SIP在不同大肠组织中的表达及临床意义  

Clinical significance of expression of CacyBP/SIP in colorectal tissues

作　　者：翟惠虹[1] 冯珊珊[2] 仇长青[1] 武婧[1] 王安萍[1] 

机构地区：[1]宁夏医科大学总医院消化内科,宁夏回族自治区银川市750004 [2]宁夏医科大学总医院外科学研究室,宁夏回族自治区银川市750004

出　　处：《世界华人消化杂志》2014年第9期1328-1333,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81072040~~

摘　　要：目的:检测钙周期素结合蛋白[calcyclin(S100-A6)-binding-protein,CacyBP/Siah-1 interactingprotein,SIP]在大肠各种组织中的表达,探讨其在大肠癌发生发展中的作用.方法:采用免疫组织化学技术检测10例正常大肠组织、17例大肠增生性息肉、26例大肠腺瘤和50例大肠癌组织中CacyBP/SIP的表达.采用Western blot检测3例大肠癌及相应癌旁正常组织中CacyBP/SIP的表达.并分析CacyBP/SIP与大肠癌临床病理资料之间的关系.结果:CacyBP/SIP在正常大肠、大肠增生性息肉、大肠腺瘤及大肠癌组织中的表达率分别为0(0/10)、17.7%(3/17)、26.9%(7/26)、52.0%(26/50),CacyBP/SIP在大肠增生性息肉中的表达与正常大肠组织中的表达比较无统计学差异(P>0.05),而在大肠腺瘤组织、大肠癌组织中的表达与正常大肠组织比较差异均有统计学意义(P<0.05).Western blot显示CacyBP/SIP在大肠癌组织中的表达明显高于其相应癌旁正常组织,差异有统计学意义(P<0.05).CacyBP/SIP在大肠癌组织中的表达与大肠癌患者的性别、年龄、组织分化程度、TNM分期之间比较均无统计学差异(P>0.05).结论:CacyBP/SIP在大肠腺瘤组织、大肠癌组织中高表达,可能参与了大肠癌的发生发展.AIM： To detect the expression of CacyBP/SIP [Calcyclin （S100A6）-binding protein/Siah-1 interacting protein] in normal colorectal tissue, colorectal hyperplastic polyps, colorectal adeno- ma and colorectal cancer. METHODS： Immunohistochemistry was used to analyze the expression of CacyBP/SIP in 10 normal colorectal tissue samples, 17 samples of colorectal hyperplastic polyps, 26 samples of colorectal adenomas and 50 samples of colorec- tal cancer. Four frozen tissue samples of colorec-tal cancer and adjacent noncancerous tissues were used in Western blot to detect CacyBP/SIP expression. Immunohistochernical results were compared against patient data and pathological analysis of tissue slices. Slices were assessed for associations with gender, age, degree of tumor differentiation and TNM stage. RESULTS： CacyBP/SIP was detected in 0 （0/10） of normal tissue samples, 17.7% （7/26） of colorectal hyperplastic polyps, 26.9% （7/26） of colorectal adenomas, and 52% （26/50） of colorectal cancer tissues by immunohistochem- istry. The expression of CacyBP/SIP in colorec- tal adenomas and colorectal cancer tissues was higher than that in the normal colon tissue and colorectal hyperplasic polyps （0 vs 26.9%, 0 vs 52%, 17.7% vs 26.9%, 17.7% vs 52%, P 〈 0.05 for all）. The expression of CacyBP/SIP was also higher in cancerous tissue than in adjacent non- cancerous colorectal tissue, as revealed by West- ern blot （P 〈 0.05）. There was no association be- tween CacyBP/SIP expression and patient age, gender, degree of tumor differentiation or TNM stage （P 〉 0.05 for all）. CONCLUSION： These results suggest that Ca- cyBP/SIP may be involved in the progression of colorectal cancer.

关 键 词：CacyBP SIP 大肠癌 免疫组织化学 蛋白质免疫印迹 

分 类 号：R735.34[医药卫生—肿瘤]