孔雀石绿及其代谢物在杂交鳢(斑鳢♀×乌鳢♂)体内的残留及消除规律研究  

Residue elimination of malachite green and its primary metabolite in hybrid snakehead( Channa maculata♀ × Channa argus ♂ )

作　　者：刘书贵[1] 郑光明[1] 尹怡[1] 王群[2] 戴晓欣[1] 宋怿[2] 刘毅辉[1] 马兵[2] 

机构地区：[1]中国水产科学研究院珠江水产研究所/农业部水产品质量安全风险评估实验室(广州),广东广州510380 [2]中国水产科学研究院,北京100141

出　　处：《中国渔业质量与标准》2014年第1期26-32,共7页Chinese Fishery Quality and Standards

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(2012A1103)

摘　　要：本实验模拟自然养殖条件,对杂交鳢(斑鳢Channa maculata♀×乌鳢Channa argus♂)肌肉中的孔雀石绿(MG)及其代谢物隐色孔雀石绿(LMG)的残留及消除规律进行了研究。对池塘中初始体重为(200±10)g的杂交鳢进行质量浓度为200μg/L的MG药浴,药浴后于0.5、1、2、4、8、12、24、48、96、192、288、384、480、720、960、1 200、1 440、1 920、2 400、2 880、3 600、4 320、5 040和5 760 h取样,采用液相色谱串联质谱法(HPLC-MS/MS)测定杂交鳢肌肉组织中MG和LMG的残留量。药浴0.5~96 h期间,MG和LMG在杂交鳢肌肉中的残留量不断波动式升高,分别达到(543.77±22.14)μg/kg和(220.51±7.93)μg/kg。MG从96 h之后呈现下降趋势,到1 920 h后MG残留量低于检测限0.5μg/kg。而LMG在288~480 h范围内的残留量基本维持在(73.72±4.85)^(70.33±9.27)μg/kg之间,1 920h时LMG的残留量急剧下降至(2.25±0.25)μg/kg,至5 040 h已低于检测限0.5μg/kg。结果表明,MG和LMG在杂交鳢体内的残留消除期远大于水体,且LMG比MG代谢时间长。The residues and depuration of malachite green （MG） and its primary metabolite leuco - malachite green （LMG）in hybrid snakeheads（ Channa maculata ~ x Channa argus c3 ）were studied. The average weight of hybrid snakeheads was about（200 -＋ 10）g , and they were exposed to 200 μg/L MG in a 667 m2pond. The samples were collected at 0.5, 1,2, 4, 8, 12, 24, 48, 96, 192, 288, 384,480,720, 960, 1 200, 1 440,1 920, 2400, 2 880, 3 600, 4 320, 5 040, and 5 760 h respectively, and the residues of MG and LMG were tested by high performance liquid chromatography tandem mass spectrometry（ HPLC -MS/MS）. The residues of MG and LMG increased in muscle unceasingly from 0.5 to 96 h and were up to（543.77 ±22.14） μg/kg and（220.51 ±7.93） μg/kg , respectively. The residues of MG decreased after 96 h, and were below the detection limit of 0.5 μg/kg at 1 920 h. The residue of LMG maintained between （73.72 ± 4.85 ） and （70.33±9.27 ） p,g/kg from 288 to 480 h, while significantly decreased to （2.25 ±0.25 ） μg/kg at 1 920 h, and was below the detection limit of 0.5 μg/kg at 5 040 h. The results also indicated that LMG was more slowly degradated than MG, which could be used as a marker to determine whether MG was used in aquaculture. [ Chinese Fishery Quality and Standards, 2014, 4（ 1 ）：26- 32 ]

关 键 词：杂交鳢 孔雀石绿 隐色孔雀石绿 残留 消除 

分 类 号：S948[农业科学—水产养殖]