替米沙坦通过上调PPAR-γ抑制肺腺癌A549细胞MMP-7表达的实验研究  

Telmisartan Inhibits through Up-regulation of PPAR-γ in A549 Cells

作　　者：任素蓉[1] 廖垚[1] 周筱秋[1] 王晋[1] 胥云霞[1] 

机构地区：[1]绵阳市中心医院肿瘤科,四川绵阳621000

出　　处：《四川医学》2014年第3期301-303,共3页Sichuan Medical Journal

摘　　要：目的探讨替米沙坦能否通过上调PPAR-γ抑制肺腺癌A549细胞MMP-7表达。方法使用替米沙坦对A549细胞进行干预。在不同时间点(0 h,24h和72h)使用MTT法对A549细胞活性进行测定。使用PT-PCR对在不同时间点(0 h,24h和72h)细胞表达的MMP-7和PPAR-γmRNA进行测定。使用western blotting对在不同时间点(0 h,24h和72h)细胞表达的MMP-7和PPAR-γ蛋白进行测定。结果替米沙坦干预后A549细胞活性逐渐降低,明显低于对照组(P均<0.05),且各时间点间的细胞活性差异有统计学意义(P均<0.05)。不同时间点(0 h,24h,和72h)MMP-7mRNA和蛋白的表达呈时间依赖性的递减(P均<0.05),而PPAR-γmRNA和蛋白的表达呈时间依赖性的递增(P均<0.05)。结论本实验表明替米沙坦可能是通过上调PPAR-γ的表达抑制MMP-7的合成和分泌导致A549细胞生长和侵袭能力的降低。Objective To investigate whether telmisartan ＇would inhibit MMP-7 via up-regulation of PPAR-γ in A549 Cells. Methods MTr assay was used to measure the cell viability. RT-PCR was included to analyze MMP-7 and PPAR-3, mR- NA. The protein expression of MMP-7 and PPAR-γ was measured by western blotting. Results After telmisartan intervention, the cell viabilities of A549 were time dependently decreased.. Then, the mRNA and protein expression of MMP-7 was reduced by telm- isartan in a time dependent manner. Meanwhile, the mRNA and protein expression of PPAR-γ was time dependently up-regulated by telmisartan. Conclusion Telmisartan could reduce MMP-7 probably through up-regulation of PPAR-γ in A549 cells.

关 键 词：替米沙坦 MMP-7 PPAR-Γ A549细胞 

分 类 号：R734.2[医药卫生—肿瘤]