没食子酸体外诱导人肝癌SMMC-7721细胞凋亡的机制研究  

Study of Gallic Acid in Inducing Human Hepatoma SMMC-7721 Cells Apoptosis and Its Mechanism

作　　者：李沐涵[1] 王明艳[1] 陈海彬[1] 周红光[1] 李文婷[1] 吴勉华[1] 

机构地区：[1]南京中医药大学,江苏南京210023

出　　处：《中药新药与临床药理》2014年第2期117-121,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家科技部973计划中医理论专项课题(2006CB504807);国家自然科学基金项目(30973742;81273717);国家自然科学青年基金项目(81102563);教育部博导课题(20113237110001)

摘　　要：目的研究没食子酸（gallicacid，GA）体外诱导人肝癌SMMC-7721细胞凋亡及其作用机制。方法体外培养人肝癌SMMC-7721细胞，噻唑蓝（M1Yr）法观察细胞增殖；Hoechst-33258、AnnexinV—FITC／PI检测细胞凋亡情况；逆转录聚合酶链式反应（RT—PCR）法研究凋亡抑制基因SurvivinmRNA的变化。结果6．25～50μmol·L-4GA可抑制SMMC-7721细胞的生长，并呈剂量依赖性；不同剂量的GA作用48h后，SMMC-7721细胞出现明显的凋亡形态，细胞凋亡率有显著的剂量依赖性。RT—PCR法检测结果显示，GA能明显抑制肝癌细胞中SurvivinmRNA的表达。结论GA可抑制人肝癌SMMC-7721细胞的增殖，这可能与下调凋亡抑制基因Survivin有关。Objective To investigate the inhibition of gallic acid（GA） on growth of human hepatoma SMMC-7721 cells in vitro and to explore its potential mechanism. Methods SMMC-7721 ceils were cuhured in vitro. Methyl thiazolyl tetrazolium （MTlP） assay was used to observe the growth of SMMC-7721 cells. Apoptosis of the cells was analyzed by Hoeehest-33258 staining. Annexin V-FITC/PI staining was used to quantify the percentages of apoptotie cell in the total cell population. Survivin mRNA expression was investigated by reverse transcription polymerase chain reaction （RT-PCR）. Results The results of MTF assay showed that 6.25 -50 μmol L-l of GA could significantly inhibit the proliferation of SMMC-7721 cells in dose-dependent manner. Hoeehest-33258 staining results presented obvious apoptotic morphology of SMMC-7721 cells treated with GA at various concentrations for 48 h. The results of Annexin V-FITC/PI double-staining indicated that the apoptotic rate increased significantly with GA in dose-dependent manner. RT-PCR results showed that the expression of Survivin mRNA declined in GA groups. Conclusion GA can inhibit the growth of human gastric cancer cells and induce cells apoptosis. Its mechanism may be associated with down-regulating survivin mRNA expression.

关 键 词：没食子酸 SMMC-7721细胞 细胞凋亡 SURVIVIN 

分 类 号：R285.5[医药卫生—中药学]