几丁糖对成纤维细胞增殖及Ⅲ型前胶原信使核糖核酸表达的影响  

Effect of chitosan on fibroblastic proliferation and type Ⅲ procollagen mRNA expression

作　　者：宋健[1] 朱樑[1] 张兴荣[1] 沈建伟[1] 张贤康[1] 

机构地区：[1]中国人民解放军第二军医大学附属长征医院消化科,上海200003

出　　处：《中国新药与临床杂志》2001年第1期53-55,共3页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的 :研究几丁糖对成纤维细胞增殖及Ⅲ型前胶原mRNA表达的影响 ,阐明其抗纤维化的作用机制。方法 :用四唑盐 (MTT)比色法及RNA斑点杂交分别检测NIH /3T3细胞增殖及Ⅲ型前胶原mRNA的表达。结果 :几丁糖能明显抑制成纤维细胞增殖及Ⅲ型前胶原mRNA的表达 ,并呈剂量依赖性。结论AIM: To study the effect of chitosan on fibroblastic proliferation and procollagen Ⅲ mRNA expression in searching of the effective mechanism against hepatic fibrosis. Chitosan is a product of depolymerization and deacetylation of chitin, which has extensive biofunction because of its complex constructions. METHODS: The NIH/3T3 fibroblastic proliferation and type Ⅲ procollagen mRNA expression were studied with MTT cololimetry and RNA blot hybridizaiton. RESULTS: Chitosan could significantly inhibit fibroblastic proliferation and type Ⅲ procollagen mRNA expression and showed dose dependent. CONCLUSION: The chitosan is an effective nature medicine for anti liver fibrosis, through inhibitting fibroblastic proliferation and type Ⅲ procollagen mRNA expression.

关 键 词：几丁糖 成纤维细胞 纤维变性 

分 类 号：R575.205[医药卫生—消化系统] R975[医药卫生—内科学]