HPLC法测定稀戊二醛溶液中戊二醛含量  

Determination of Glutaraldehyde in Dilute Glutaraldehyde Solution by HPLC

作　　者：杜建红[1] 徐小平[2] 王艳丽[1] 郄革红[1] 赵晓娟[1] 

机构地区：[1]成都军区联勤部药品仪器检验所,成都610017 [2]四川大学华西药学院,成都610041

出　　处：《北方药学》2014年第4期2-3,共2页Journal of North Pharmacy

基　　金：军队医疗机构制剂标准提高科研专项课题面上项目;编号:13ZJZ04-2

摘　　要：目的：建立HPLC法测定稀戊二醛溶液中戊二醛含量的方法。方法：采用AichromBond-AQ C18色谱柱（4.6mm＆#215;150mm，5μm），流动相：甲醇∶水（50∶50），流速0.5ml/min，检测波长235nm，进样量20μl。结果：戊二醛在51.6～1010.4μg/ml范围内时，与峰面积呈良好的线性关系（r=0.9992），平均回收率为101.1%。结论：本法操作简便，灵敏度高，重复性好，可用于稀戊二醛溶液中戊二醛的含量控制。Objective：To establish an HPLC method for the determination of Glutaraldehyde in Dilute Glutaraldehyde Solution.Method：An AichromBond-AQ C18 column（4.6mm＆#215;150mm,5μm） was used,the mobile phase was Methanol-Water（50∶50）.The flow rate was 0.5 ml/min,the detection wavelength was 235nm and the injection volume was 20μl. Results： Within the range from 51.6 to 1010.4μg/ml,Glutaraldehyde presented a good relationship between the injected amounts and peak areas （r=0.9992）.The average recovery was 101.1%.Conclusion： The method was easy to operate, with sensitive,and reproducible. It can be used for the quality control of Glutaraldehyde in Dilute Glutaraldehyde Solution.

关 键 词：稀戊二醛溶液 戊二醛 高效液相色谱法 测定 

分 类 号：R927.2[医药卫生—药学]