星点设计–效应面法优化川芎提取工艺  

Optimization of extraction process of Ligusticum chuanxiong by central composite design-response surface method

作　　者：吕维[1] 吴恋[1] 罗红丽[1] 叶娜[1] 王春艳[1] 范成杰[1] 万丽[1] 

机构地区：[1]成都中医药大学药学院,中药材标准化教育部重点实验室,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都610075

出　　处：《药物评价研究》2014年第1期53-57,共5页Drug Evaluation Research

摘　　要：目的采用星点设计效应面法优化川芎中阿魏酸和藁本内酯的提取工艺。方法以川芎药材中阿魏酸、藁本内酯的质量分数为考察指标,考察乙醇体积分数、提取温度以及提取时间对提取工艺的影响,对结果进行二项式拟合,用效应面法选择较佳工艺条件,并进行预测分析。结果确定较优提取工艺为83℃下,14倍量73%乙醇提取2次,每次2 h,含量实际值与预测值偏差为-1.37%。结论星点设计效应面法优化川芎提取工艺,方法简便,结果可靠,预测性良好。Objective To optimize the extraction process of ferulaic acid and ligustilide in Ligusticum chuanxiong by central composite design and response surface method. Methods The effects of ethanol concentration, extracting temperature, and extracting time on the contents of ferulaic acid and ligustilide were investigated by using central composite design and response surface method. The data were imitated using multi-linear equation and second-order polynomial equation. Results The optimal conditions of extraction process were 14 fold solvent, 2 h for decoction, and extracting twice; The ethanol concentration was 73%, the extracting temperature was 83 ℃. The variance between observed and predicted values was -1.37%. Conclusion The central composite design and response surface method is convenient, reliable, and highly predictive.

关 键 词：川芎 阿魏酸 藁本内酯 效应面法 提取工艺 

分 类 号：R943.4[医药卫生—药剂学]