不同方法糖化玉米秸秆的工艺优化  

Process Optimization of Corn Straw Saccharification by Different Methods

作　　者：郭海鹏[1] 李秀欣[1] 马海燕[1] 田亚红[1] 

机构地区：[1]河北联合大学生命科学学院,河北唐山063000

出　　处：《河南农业科学》2014年第4期137-140,151,共5页Journal of Henan Agricultural Sciences

基　　金：河北省科技厅科技支撑项目(12227107D)

摘　　要：以玉米秸秆为原料,分别研究酶解糖化法和生物糖化法的糖化效果,以找出最优糖化工艺。结果表明,纤维素酶糖化玉米秸秆的最佳工艺条件为时间40h、纤维素酶量1 400U/g、温度30℃、pH值4.8,在此条件下的产糖量为73.4mg/g;黑曲霉糖化玉米秸秆的最佳工艺条件为发酵时间48h、接种量13%、发酵温度35℃、pH值4.8,在此条件下的产糖量为69.7mg/g。2种方法产糖量相差不多,从成本考虑,建议采用生物糖化法。The corn straws were saccharified by enzymolysis method and biological fermentation method respectively to find the optimum corn straw saccharification process. The results showed that the optimum conditions of enzymolysis with cellulase were hydrolysis time of 40 h,cellulase dosage of 1 400 U/g,temperature of 30 ℃ ,pH value of 4.8,and the reducing sugar yield was 73.4 mg/g under the above conditions. The optimum conditions of fermentation with Aspergillus niger were fermentation time of 48 h, inoculation amount of 13%, fermentation temperature of 35 %C, pH value of 4.8, and the reducing sugar yield was 69.7 mg/g under the above conditions. The sugar yields of the two methods were very similar, but the biological fermentation method was recommended based on the cost.

关 键 词：玉米秸秆 纤维素酶 黑曲霉 糖化 

分 类 号：TQ223.122[化学工程—有机化工]