原发性膀胱移行细胞癌Pgp与突变p53蛋白、EGFR的相互关系  

EXPRESSION OF P-GLYCOPRITEIN AND MUTANT P53, EPIDERMAL GROWTH FACTOR RECEPTOR IN HUMAN PRIMARY TRANSITIONAL CELL CARCINOMAS OF BLADDER

作　　者：周辉良[1] 罗义麒[1] 曹林升[1] 张声[2] 

机构地区：[1]福建医科大学附属第一医院泌尿外科,福州350005 [2]福建医科大学附属第一医院病理科

出　　处：《中国现代医学杂志》2001年第2期28-30,共3页China Journal of Modern Medicine

摘　　要：目的 :探讨mdr1基因表达产物Pgp与突变 p5 3蛋白、EGFR是否存在相关性及其临床意义。方法 :采用免疫组化SP法 ,检测 10 7例原发性膀胱移行细胞癌中Pgp、突变 p5 3蛋白、EGFR表达情况。结果 :Pgp、突变p5 3蛋白、EGFR的阳性表达率分别为 6 3 .6 % ,72 .9%和 6 6 .4% ,三者的过表达均与膀胱癌的病理分级、分期和术后的复发有关 ;相关性研究显示Pgp表达与突变 p5 3蛋白和EGFR的表达密切相关 (P <0 .0 1)。结论 :Pgp、突变 p5 3蛋白和EGFR过表达不仅是判定原发性膀胱移行细胞癌恶性程度和预后的重要指标 ,而且提示原发性膀胱移行细胞癌中Pgp的表达可能与其他肿瘤一样 ,受到 p5 3和EGFR等的调控。Objective:The study investigates clinical significance and the interrelationship between expression of multi-drug resistance gene (MDR1) product (P-glycoprotein Pgp)and mutant p53,epidermal growth factor receptor (EGFR) in human primary bladder transitional cell carcinomas(TCCs).Methods:107 human primary bladder TCCs of previously untreated patients were analyzed for expression of Pgp,Mutant p53 and EGFR by means of SP immunohischemistry (IHC).The mouse's monoclonal antibodies of JSB1,DO-7 and 31G7 were carried out to detected the antigens of Pgp,p53 and EGFR,respectively.Results:Pgp,p53 and EGFR were present in 63.6%,72.9% and 66.4% of TCCs.The expressions of Pgp,p53 or EGFR were significantly correlated with the grad,stage and recurrence after post-operative intravesical chemotherapy.The expressions of Pgp were strongly related to the expression of mutant p53 and EGFR (Pgp/p53,P<0.01;Pgp/EGFR,P<0.01).Conclusions:Overexpression of Pgp,mutant p53 and EGFR can be used as the important markers of aggressiveness and prognosis of TCCs.The expression of Pgp could be modulated by mutant p53 and EGFR,as is found in other tumors.

关 键 词：膀胱肿瘤 移行细胞癌 P糖蛋白 P53表皮生长因子受体免疫组化 

分 类 号：R737.14[医药卫生—肿瘤]