当归配方颗粒质量分析研究  

Study on the Quality of DangGui Granules

作　　者：吴志成[1,2] 张泽国[3] 徐福菊 李越峰[3] 

机构地区：[1]甘肃中医学院附属医院,甘肃兰州730000 [2]甘肃省中药炮制及质控工程技术研究中心 [3]甘肃中医学院

出　　处：《西部中医药》2014年第4期13-17,共5页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(编号81073077);教育部科学技术研究重点项目(编号212186);甘肃省财政厅高校基本科研业务费专项资金(编号2013-2);2010年度甘肃省自然科学基金(编号1010RJZA212);2010甘肃中医学院引进人才科研启动基金(编号2010-3)

摘　　要：目的：测定当归配方颗粒中阿魏酸含量、水分和浸出物含量，为科学评价及有效控制其质量提供可靠方法。同时，进行当归配方颗粒及当归饮片的质量比较。方法：提取方法、色谱方法均在2010版（《中国药典》基础上进行了优化。高效液相色谱法，色谱柱：KromasilC18色谱柱（4．6mm×250mm,5μm），流速：1．0mL／min，检测波长：316nm，柱温：28℃，流动相：乙腈-0．05％磷酸水梯度洗脱系统。结果：阿魏酸在0．033-0．165μg忙0．9998，n=5）之间有良好的线性关系，平均加样回收率为98．78％，RSD=0．99％。结论：当归配方颗粒在各项含量指标上都符合当归的质量要求，阿魏酸含量高于当归饮片。采用HPLC方法测定当归配方颗粒中阿魏酸的含量，操作简单，可靠，重现性好，可作为当归配方颗粒质量控制的有效方法。Objective： To provide reliable methods for scientific evaluation and effective control of the quality of Dangui （angelic sinensis） granules by determining the contents of ferulic acid, moisture and the contents of ex- tracts in the granules. Meanwhile, to compare the quality of Dangui granules and its prepared slices. Methods： The extracting method and chromatographic process were different from the methods in pharmacopeia of 2010 edition, which were the optimization of the methods in pharmacopeia. HPLC was adopted, chromatographic column： Kro- masil C18 （4.6 min×250 mm, 5 μm）, flow rate： 1.0 mL/min, detection wavelength：316 nm, the temperature：28 ℃, mo- bile phase： acetonitrile-0.05% phosphoric acid in water gradient elution system. Results： Ferulic acid showed better linear relationship in the range between 0.033 and 0.165 μg （r=0.999 8, n=5）. Average recovery rate was 98.78%, RSD=0.99%. Conclusion： Dangui granules could meet quality requirement of Dangui in the contents of different in- dexes, the contents of ferulic acid are higher than these in prepared slices of Dangui. Meanwhile, according to the re- sults, it is simple, reliable and reproducible to determine the contents of ferulic acid in Dangui granules by HPLC, which is an effective method for quality control of Dangui granules.

关 键 词：当归配方颗粒 阿魏酸 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]