HPLC法同时测定藏药岷县龙胆中4种有效成分的含量  

Simultaneous Determination of Four Active Components in Tibetan Herb Gentiana purdomii Marq. by HPLC

作　　者：董琦[1] 吉文鹤[1] 肖远灿[1] 谭亮[1] 胡风祖[1] 

机构地区：[1]中国科学院西北高原生物研究所,西宁810008

出　　处：《天然产物研究与开发》2014年第4期561-563,共3页Natural Product Research and Development

基　　金：青海省自然科学基金项目(2011-Z-922Q)

摘　　要：采用高效液相色谱法同时测定藏药岷县龙胆中獐牙菜苦苷、龙胆苦苷、獐牙菜苷和异荭草苷含量。结果测定岷县龙胆中獐牙菜苦苷、龙胆苦苷、獐牙菜苷和异荭草苷的含量分别为0.108%、1.46%、2.21%、1.61%。色谱条件为:色谱柱:XDB-C18(4.6 mm×250 mm,5μm);柱温:25℃;流速:1.0 mL/min;流动相:甲醇/0.01%乙酸水溶液梯度洗脱;检测波长260 nm。本方法快速、准确、重复性好,适用于岷县龙胆中4种有效成分的含量测定,可为藏药岷县龙胆药材的质量控制提供依据。A quantitative method of the simultaneous determination of swertiamain, gentiopicroside, sweroside and Isoori-entin in C, entiana purdomii Marq. was established using RP-HPLC. The results showed that the contents of swertiamain, gentiopicroside, sweroside and Isoorientin were 0. 108%, 1.46% ,2.21% and 1.61%, respectively. The HPLC analysis conditions were as follows：XDB-C18 column （4.6mm×250mm,5μm） was used for chromatographic separation and methanol-0.01% lacetic acid were used as the mobile phases with gradient elution;The flow rate was 1.0 mL/miu with a column temperature at 25 ℃ ,and the UV detection wavelength was set at 260 nm. The assay demonstrated that the de-veloped method had adequate accuracy and selectivity. It was suitable for the determination of four active components in G. purdomii. Hence ,it can provide additional evidence for the quality evaluation of G. purdomii.

关 键 词：岷县龙胆 苦苷类成分 异荭草苷 HPLC 

分 类 号：R29[医药卫生—民族医学]