复方枳葛橘提取物体外抗氧化活性评价(英文)  

Antioxidant Activity of the Extracts of Formulation Zhigeju

作　　者：陈地灵[1] 李宁[1] 林励[2] 张鹤鸣[1] 林辉[2] 刘颂豪[1] 

机构地区：[1]华南师范大学药物研究院,广州510631 [2]广州中医药大学,广州510006

出　　处：《天然产物研究与开发》2014年第4期601-605,618,共6页Natural Product Research and Development

基　　金：China National Ministry of Science and Technology Plan Projects(2011BAI01B02);Guangdong Science and Technology Plan Projects(2012A030100005)

摘　　要：复方枳葛橘水提液依次用氯仿、乙酸乙酯和正丁醇萃取,与水层得到4个不同极性部位萃取物,以清除DPPH·、ABTS+·,以及络合Fe2+能力,综合评价其抗氧化作用;采用芦丁显色法检测各部位总黄酮含量,并分析黄酮类物质含量与抗氧化活性的相关性;采用HPLC法分析其抗氧化能力较强部位可能的化学成分。结果显示4个不同极性部位均具有不同程度的抗氧化能力,其中乙酸乙酯部位抗氧化能力最强,其次是正丁醇、水层,而氯仿层较弱;其抗氧化能力与总黄酮含量呈正相关,且与黄酮类化合物结构类型相关;HPLC分析结果显示乙酸乙酯合并正丁醇部位主要成分为柚皮苷、野漆树苷和槲皮素等黄酮类化合物。复方枳葛橘提取物可以作为天然抗氧化剂用于药物研究和相关疾病的防治。The formulation Zhigeju was fractionated by using 3 different organic solvents and water to provide chloroform extract （ CE）, ethyl acetate extract （ EAE）, n-butanol extract （BE） and water extract （WE）. Different antioxidant as-says （DPPH, ABTS scavenging activity and metal chelating activity） were employed to evaluate the antioxidant activities of the four extracts. The contents of total flavonoids in different extracts were measured through UV spectroscopy using rutin as control by color reaction. The correlation between the concentration of total flavonoids and their antioxidant activ-ities was then investigated. HPLC was used to analysis the components of the extract with the highest antioxidant activi- ty. The results showed that the EAE had the highest antioxidant activity while the CE had the lowest antioxidant activity. All of the four extracts showed concentration dependent antioxidant activity,however the IC50 values of the four extracts on antioxidant activity were different. The results of HPLC analysis showed that the main components of EKE and BE were flavonoids,ineluding naringin, rhoifolin and quercitin. The extracts of formulation Zhigeju had the potential to be used as natural antioxidant for the drug research and related disease prevention.

关 键 词：复方枳葛橘 提取物 自由基 总黄酮 抗氧化活性 

分 类 号：R285.5[医药卫生—中药学]