二十五味珊瑚丸对D-半乳糖衰老鼠海马细胞形态和GFAP表达的影响  

Effects of 25 Odors Coral Pills on Pyramidal Cell and the Expression of Glial Fibrillary Acidic Protein in Hippocampus of D-Galactose Induced Brain Aging Rats

作　　者：李鹏[1] 杨春[1] 黄福开[2] 罗远带[2] 胡贤达 邵杰[2] 王艳[2] 刘亚丽[3] 原林[1] 

机构地区：[1]南方医科大学人体解剖学教研室暨广东省组织构建与检测重点实验室,广州510515 [2]中国藏学研究中心北京藏医院,北京100029 [3]南方医科大学中医药学院,广州510515

出　　处：《天然产物研究与开发》2014年第4期606-609,共4页Natural Product Research and Development

基　　金：国家重大财政专项:藏医心脑血管临床研究与药物研发(1981020421);省产业技术研究与开发资金项目(2011 B010500004)

摘　　要：探讨二十五味珊瑚丸对D-半乳糖衰老模型大鼠海马锥体细胞形态和星形胶质细胞胶质纤维酸性蛋白(GFAP)表达的影响。采用颈背部皮下注射D-半乳糖制作大鼠衰老模型。于造模的第6周,给予二十五味珊瑚灌胃。之后取各组脑组织进行HE染色、GFAP免疫组化染色以及Western blotting。与模型组比较,二十五味珊瑚丸组大鼠海马CA3区锥体细胞层细胞丢失较少,细胞排列较紧密、整齐;DG区GFAP阳性的星形胶质细胞数量减少,胞体较小,突起较细;海马GFAP表达下降,差异有显著性(P<0.05)。D-半乳糖衰老模型大鼠海马锥体细胞衰老变性且GFAP的表达增多;二十五味珊瑚丸可抑制D-半乳糖衰老模型大鼠海马锥体细胞的衰老变性和星形胶质细胞GFAP的表达。The D-galactose induced brain aging model was used to study the effect of 25 odors coral pills on hippocam-pus. The morphology of pyramidal cells and the expression of glial fibrillary acidic protein （GFAP） in hippocampus were determined in each experimental group. Hippocampal neuronal degeneration on CA3 region was observed by hematoxylin-eosin （HE） staining,the expression of GFAP was explored by immunohistochemistry,Westem blotting and densitometric analysis. The results showed that compared with model group,the degeneration of hippocampal pyramidal ceils on CA3 in 25 odors coral pills group was significantly improved and the number of cells positive for GFAP and the expression of GFAP in the hippocampus were significantly reduced. These results indicated that 25 odors coral pills inhibited hipp-ocampal neuronal degeneration and the GFAP expression in the D-galactose induced brain aging rats.

关 键 词：二十五味珊瑚丸 D-半乳糖 衰老 锥体细胞 胶质纤维酸性蛋白 

分 类 号：R741[医药卫生—神经病学与精神病学]