纯合子红细胞抗原在鉴定低效价抗体中的应用  

Study on identification of low titer antibody using homozygous red cell antigen

作　　者：马红丽[1] 王志红[1] 李小峰[1] 

机构地区：[1]洛阳市中心血站,河南洛阳471000

出　　处：《中国输血杂志》2014年第3期261-263,共3页Chinese Journal of Blood Transfusion

基　　金：洛阳市科技发展计划项目(No.1101053A)

摘　　要：目的探讨纯合子红细胞抗原在低效价不规则抗体鉴定中的意义。方法针对有剂量效应的不规则抗体(抗-D、抗-E、抗-c、抗-C),制备相应的纯合子红细胞和杂合子红细胞,以盐水法、凝聚胺法、抗球蛋白试验,分别鉴定待测血清标本中相应抗体效价,观察各自的效价评分。结果与杂合子组相比,纯合子组抗球蛋白试验的效价评分轻度升高,凝聚胺试验的效价评分显著升高。结论纯合子红细胞能有效鉴定低效价不规则抗体,确保临床输血的安全性和有效性。Objective To explore the significance of applying homozygous RBC in identifying low titer irregular antibod-ies. Methods The homozygous and heterozygous RBC were identify irregular antibody （ anti-D, anti-E, anti-e, anti-C） by brine method,polybrene, and, eoomb＇ s test, respectively. Then integral was calculated to analyze the titer. Results Com-pared with heterozygous RBC,the integral significantly increased after treatment with homozygous RBC by polybrene test, and the value of coomb＇s test was slightly elevated. Conclusion Applying homozygous RBC can identify low titer irregular anti-bodies effectively which could ensure the safety and efficacy of clinical blood transfusion.

关 键 词：纯合子 红细胞抗原 低效价抗体 

分 类 号：R457.11[医药卫生—治疗学]