Cks1、Skp2和p27在人脑胶质瘤中的表达及相互关系  

Expressions of Cksl, Skp2 and p27 in human gliomas and their correlations

作　　者：沈晓燕[1] 房晓[1] 蒋磊[1] 盛平[1] 丁茂华[1] 仇冠中[1] 吴小军[1] 胡国汉[1] 

机构地区：[1]第二军医大学附属长征医院神经外科、上海市神经外科研究所,上海200003

出　　处：《中华神经医学杂志》2014年第4期336-339,共4页Chinese Journal of Neuromedicine

摘　　要：目的研究周期素依赖性蛋白激酶亚单位1（Cks1）、S期激酶相关蛋白2（Skp2）和周期素依赖性蛋白激酶抑制物p27在人脑胶质瘤中的表达及相互关系，同时探讨Cks1对胶质瘤恶性生物学进展的可能作用机制。方法收集上海长征医院神经外科自2009年6月至2011年6月手术切除的胶质瘤组织标本34例及颅脑外伤内减压术中切除的非肿瘤脑组织10例，运用免疫组化EnVision法检测Cks1、Skp2和p27的蛋白表达水平并比较相互关系。结果正常脑组织、低级别（WHOⅠ、Ⅱ级）和高级别（WHOⅢ、Ⅳ级）胶质瘤中Cks1蛋白表达的免疫反应评分（IRS）分别为4．90±2，38，3．20±2．98和2．16±2．52，随胶质瘤病理级别进展表达下降，三者差异有统计学意义（P〈0．05）。Skp2在正常脑组织中无表达，低级别和高级别胶质瘤中的1RS为1．00±1．13和2．23±2.23，随胶质瘤病理级别进展表达增加，差异有统计学意义（P〈0．05）。正常脑组织中p27表达IRS为7．80±0．63，低级别和高级别胶质瘤中IRS分别为5．00±2．10和2．21±1．47，差异有统计学意义（P〈0．05）。34例胶质瘤组织标本中，Cks1表达与p27表达呈正相关（r=0．380，P=0．027），Skp2表达水平与Cks1、p27均无相关性（r=0．080，P=0．662；r=-0．160，P=-0．376）。结论Cks1、Skp2和p27可作为评价胶质瘤良恶性程度的参考指标；Cks1可能通过促进p27蛋白聚集起到抑制脑胶质瘤恶性进展作用．Objective To investigate the expressions ofcyclin kinase subunit 1 （Cks1）, S-phase kinase associated protein 2 （Skp2） and Cyclin- dependent kinase inhibitor p27 in human gliomas and their correlations, and study the possible role of Cks1 to glioma malignant progression. Methods Immunohistochemical staining （EnVision method） was used to measure the protein expressions of Cks1, Skp2 and p27 in 34 human glioma samples and 10 normal brain tissue slices, collected in our hospital from June 2009 to June 2011. Relationships between these levels of these proteins were analyzed. Results The immunoreactivity scale （IRS） scores of Cks 1 expression in normal brain tissue, low grade gliomas and high grade gliomas were 4.90±2.38, 3.20±2.98 and 2.16±2.52; Cks1 expression was decreased as the tumor grade developed. Skp2 protein was negative in all 10 cases of normal brain tissues and the IRS scores of Skp2 expression in low and high grade gliomas were 1.00± 1.13 and 2.26±2.23; they increased in a malignancy-dependent manner. The IRS scores of p27 expression in normal brain tissue, low grade gliomas and high grade gliomas were 7.80±0.63, 5.00±2.10 and 2.21 ±1.47 with significant differences （P〈0.05）. Significantly positive correlation was found only between expressions of Cks1 and p27 （r=0.38, P〈0.05）, while no significant correlations between expression of Cks1 and Skp2 and expression of p27 and Skp2 were noted （r=0.08 and -0.16, P〉0.05）. Conclusion Cks1, Skp2 and p27can be used as markers to evaluate glioma histological grade; Cks1 could inhibit gliomas malignant development by promoting p27 accumulation.

关 键 词：神经胶质瘤 周期素依赖性蛋白激酶亚单位1 S期激酶相关蛋白2 P27 免疫组织化学染色 

分 类 号：R739.41[医药卫生—肿瘤]