人脐带间充质干细胞通过激活Notch信号通路促进白血病细胞株Jurkat的体外存活(英文)  

Human umbilical cord-derived mesenchymal stem cells inhibit proliferation but maintain survival of Jurkat leukemia cells in vitro by activating Notch signaling

作　　者：袁茵[1] 陈丹亮[2] 陈璇[1] 邵红伟[1] 黄树林[1] 

机构地区：[1]广东药学院生命科学与生物制药学院//广东省生物技术候选药物研究重点实验室,广东广州510006 [2]暨南大学附属第一医院妇产科,广东广州510630

出　　处：《南方医科大学学报》2014年第4期441-447,共7页Journal of Southern Medical University

基　　金：Supported by National "Key New Drug Creation" Special and Major Project(2009ZX09103-708);National Natural Science Foundation of China(31100664,31300737,81303292);Medical Scientific Research Foundation of Guangdong Province(B2013197)~~

摘　　要：目的研究人脐带间充质干细胞对白血病细胞株Jurkat体外增殖和存活能力的影响并探讨相关机制。方法组织块贴壁法分离人脐带间充质干细胞,将其作为基质细胞与Jurkat细胞进行直接接触共培养。通过免疫磁珠法分离共培养体系中的Jurkat细胞,流式细胞术检测Jurkat细胞增殖能力、细胞周期和自发凋亡率的变化;Western blotting法检测共培养后Jurkat细胞内Notch信号通路的活化状况。结果共培养4 d后,Jurkat白血病细胞的增殖减缓、细胞周期被阻滞于G0/G1期,而其自发凋亡率则出现显著下降,并伴有Notch信号通路靶分子HES-1表达量的增加。结论人脐带间充质干细胞构成的微环境对Jurkat白血病细胞有保护作用,Notch信号通路可能参与了这一过程。Objective To investigate the effects of human umbilical cord-derived mesenchymal stem cells （hUC-MSCs） on the proliferation and survival of Jurkat leukemia cells in vitro and explore the possible mechanism. Methods Jurkat leukemia cells were cocultured with hUC-MSCs isolated from human umbilical cord tissues by plastic adherence at a ratio of 10： 1. The proliferation and survival of the co-cultured Jurkat cells, separated by immunomagnetic bead cell sorting on day 4, were evaluated by flow cytometry. Western blotting was performed to evaluate the activation of Notch signaling in the co-cultured Jurkat cells. Results Jurkat leukemia ceils co-cultured with hUC-MSCs for 4 days showed a lowered proliferation rate and cell cycle arrest at G0/G, phase with a reduction in the cell apoptotic rate. Notch signaling pathway was activated in the co-cultured Jurkat cells as evidenced by an increased cellular expression of HES-1. Conclusion Co-culture with hUC-MSCs can inhibit the proliferation of Jurkat leukemia cells in vitro and protect the cells from apoptosis by activating Notch signaling, indicating a potential shielding effect of MSCs on leukemia cells.

关 键 词：间充质干细胞 脐带 白血病 增殖 NOTCH信号通路 

分 类 号：R733.7[医药卫生—肿瘤]