热疗降低胶质瘤侵袭性作用过程中caspase 8改变的实验研究  

Experimental study of caspase 8 change in the process of hyperthermia reducing glioma invasiveness

作　　者：王健[1] 贾永森[2] 赵喜庆[3] 石文建[3] 秦丽娟[1] 

机构地区：[1]河北联合大学基础医学院生理学教研室,唐山063000 [2]河北联合大学中医学院,唐山063000 [3]河北联合大学附属唐山市人民医院神经外科,唐山063000

出　　处：《神经解剖学杂志》2014年第2期197-200,共4页Chinese Journal of Neuroanatomy

基　　金：河北省卫生厅科研基金(20100465;20110165;20120144);河北联合大学培育基金项目(SP201310)

摘　　要：目的:探讨caspase 8在热疗降低胶质瘤侵袭性过程中的作用。方法:利用Transwell构建胶质瘤侵袭模型,随机分为热疗对照组以及热疗30、60、120、180 min和240 min组。采用流式细胞术检测胶质瘤细胞凋亡水平,免疫印迹法检测胶质瘤细胞caspase 8的表达水平。结晶紫染色法测定胶质瘤侵袭性变化。结果:(1)热疗各组胶质瘤细胞凋亡率均明显高于热疗对照组(P<0.05);(2)各热疗组胶质瘤细胞的caspase 8表达均高于热疗对照组,有统计学差异(P<0.05);(3)各热疗组胶质瘤细胞的侵袭性均较对照组降低(P<0.05)。结论:热疗后胶质瘤细胞侵袭性降低,这可能与caspase 8表达增加导致胶质瘤细胞凋亡有关。Objective： To probe the role of caspase 8 in the process of hyperthermia reducing glioma invasiveness. Methods：Transwell was used to make glioma invasion models which were randomly divided into control group and hyper- thermia groups （30, 60, 120, 180 and 240 min）. Flow cytometry （FCM） was employed to test the quantification of the apoptotic glioma cells, Western-Blot was used to detect the expression of caspase 8, and crystal violet staining was used to detect the change of glioma cells invasiveness. Results： （ 1 ） The apoptotic cell rate of hyperthermia groups was significant- ly higher than that of the control group （ P 〈 O. 05 ）. （2） The caspase 8 expression in hyperthermia groups was higher than in the control group, and the difference was statistically significant （ P 〈 O. 05 ）. （3） The glioma cells invasiveness of hy- perthermia groups was lower than that of control group （ P 〈 O. 05 ）. Conclusion： Glioma cell invasion is reduced after hyperthermia, which may be related to the glioma cells apoptosis caused by the increased expression of caspase 8.

关 键 词：热疗 胶质瘤侵袭性 CASPASE8 凋亡 

分 类 号：R739.41[医药卫生—肿瘤]