胃癌患者血清热休克蛋白27、90的检测  

Detection of serum HSP27 and HSP90 in patients with gastric carcinoma

作　　者：袁建明[1] 刘涛[1] 沈建康[1] 王天翔[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院卢湾分院普外科,上海市200020

出　　处：《世界华人消化杂志》2014年第7期1000-1004,共5页World Chinese Journal of Digestology

基　　金：上海市卫计委基金资助项目;No.20134461~~

摘　　要：目的:检测胃癌患者血清热休克蛋白(heatshock protein,HSP)27、90的含量,探讨其与胃癌临床及病理指标,以及预后的关系.方法:应用ELISA方法测定42例胃癌患者血清标本中HSP27、90的含量,并与40例同期正常对照组的血清标本作对比分析.同时采用单因素方差分析和相关分析探寻HSP27、90的浓度与胃癌病理类型、病理分期及TNM分期的关系,并通过生存分析探寻其与胃癌预后的关系.结果:胃癌患者血清HSP27、90的表达显著高于正常对照者(P=0.000).在胃癌患者中,血清HSP27、90在不同病理类型、病理分期及TNM分期中均无显著差异,仅HSP27在T4组显著高于其他组(P=0.036).Pearson相关分析显示,血清HSP27和HSP90之间呈显著相关(r=0.321,P=0.038),Spearman相关分析显示,血清HSP27、90的表达与病理类型、病理分期及TNM分期无显著相关关系.Kaplan-Meier生存分析未得出血清HSP27、90和胃癌预后的相关关系.结论:血清HSP27、90表达与胃癌的发病密切相关,可作为协同判断胃癌发病的指标,且两者表达呈正相关.AIM： To detect the expression of heat shock protein（HSP） 27 and HSP90, and to investigate their correlations with clinical and pathological indexes of gastric carcinoma. METHODS： HSP27 and HSP90 levels were determined by ELISA in sera from 42 patients with gastric carcinoma and 40 healthy controls. ANOVA and Pearson or Spearman correlation analysis were used to investigate the relationship between serum levels of HSP27 and HSP90 and pathological types, pathological stage and TNM stage, and survival. RESULTS： Serum levels of HSP27 and HSP90were significantly higher in patients with gastric carcinoma than in healthy controls. Serum levels of HSP27 differed significantly between patients with T4 disease and T1-3 disease（P = 0.036）, but showed no significant correlation with pathological type, pathological stage or TNM stage. Pearson correlation analysis showed a significant relationship between serum levels of HSP27 and those of HSP90（r = 0.321, P = 0.038）. Spearman correlation analysis showed no significant relationship between serum levels of HSP27 or HSP90 and pathological type, pathological stage or TNM stage. Kaplan-Meier analysis did not show any significant relationship between serum levels of HSP27 or HSP90 and the survival rate of the patients. CONCLUSION： Serum levels of HSP27 and HSP90 may be closely related to the development of gastric carcinoma. Serum levels of HSP27 significantly correlate with those of HSP90 in patients with gastric carcinoma.

关 键 词：热休克蛋白27 热休克蛋白90 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]