基于Alu序列的分支DNA技术定量检测血游离DNA方法的建立  

Establishment of a method for quantitatively detecting circulating cell-free DNA by branch DNA-based on Alu assay

作　　者：戚菁[1] 申娴娟[1] 施维[1] 陆晶晶[1] 景蓉蓉[1] 鞠少卿[1] 吴信华[1] 

机构地区：[1]南通大学附属医院外科综合实验室,226001

出　　处：《江苏医药》2014年第7期777-779,共3页Jiangsu Medical Journal

基　　金：江苏省六大人才高峰项目(2012-WS-034)

摘　　要：目的建立一种操作简便、灵敏性较高的定量检测血游离DNA(CFD)的方法。方法运用基于Alu序列的分支DNA技术定量检测血清CFD。结果该检测的标准曲线相关系数R2=0.9916,线性范围0-400ng/ml,最低检出限0.86ng/ml,回收率93.35%-115.76%,重复性良好。正常人血清CFD水平为181.9(109.5,328.7)ng/ml,男性和女性的CFD水平差异无统计学意义(P>0.05)。结论基于Alu序列的分支DNA技术定量检测血清CFD线性较好,灵敏度较高,结果较为准确且重复性较好,易于标准化,适于临床开展大样本检测。Objective To establish a method for quantitatively detecting circulating cell-flee DNA（CFD） by branch DNA-based on Alu assay. Methods CFD was quantified using a branch DNA technique based on Alu assay. Results The correlation coefficient of standard curve （R2 ） for the branch DNA assay was 0. 9916 with the range of 0-400 ng/ml of DNA concentrations. The limit concentration of detection of the branch DNA assay was 0. 86 ng/mt. Recovery rate was in a range of 93. 35%to 115.76%. The median concentration of CFD in healthy individuals was 181.9 （109.5, 328. 7） ng/ml. There was no significant difference in CFD level between males and females （P〈0. 05）. Conclusion The method established by branch DNA based on Alu assay has a good linearity and high sensitivity in detecting CFD accurately and quantitatively, which is a suitable selection for large-scale clinical testing.

关 键 词：游离DNA Alu试验 分支DNA技术 

分 类 号：R394[医药卫生—医学遗传学]