染料木黄酮联合阿那曲唑对绝经后大鼠乳腺癌生长的影响  

Effect of Genistein Combined with Anastrozole on Mammary Tumors in Ovariectomized Rats

作　　者：王丽[1] 康欣梅[1] 宋英[1] 马文杰[1] 赵红[1] 张清媛[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院内三科哈尔滨150081

出　　处：《中国中西医结合杂志》2014年第4期486-489,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：黑龙江省普通高等学校青年学术骨干支持计划项目(No.1251G040)

摘　　要：目的利用7,12-二甲基苯蒽(DMBA)诱导大鼠绝经后乳腺癌模型,探讨染料木黄酮(Genistein)联合阿那曲唑(Anastrozole)对乳腺癌组织的抑制作用及机制。方法建立DMBA诱导的绝经后乳腺癌大鼠模型,随机分为对照组、染料木黄酮组、阿那曲唑组、染料木黄酮与阿那曲唑联合治疗组(简称联合治疗组),观察各组肿瘤生长情况,测定肿瘤增殖指数、凋亡指数;ELISA法检测雌二醇(E2)及RTPCR法检测17β-羟甾脱氢酶1(17β-HSD1)mRNA的水平。结果染料木黄酮和阿那曲唑单独治疗组肿瘤生长均受到抑制,联合治疗组的抑瘤效果更显著(P<0.05)。与对照组比较,联合治疗组的E2和17β-HSD1mRNA水平下降更明显(P<0.05)。结论染料木黄酮可抑制绝经后大鼠中乳腺肿瘤的生长,与阿那曲唑联合应用显示出降低E2和17β-HSD1水平的协同作用,可有效抑制乳腺肿瘤生长。Objective To evaluate the inhibitory effect of genistin combined with anastrozole on the growth and apoptosis of breast tumor tissue, and to study their anti-cancer mechanism by using the model of 7,12-dimethylbenz [α] anthraxcene （DMBA）-induced mammary tumors following ovariectomy in Sprague-Dawley （SD） rats. Methods The DMBA induced postmenopausal SD rats were randomly di- vided into the control group, the genistein group, the anastrozole group, and the genistein combined with anastrozole group. The growth of tumors was observed in each group. The proliferation index and apopto- sis index of tumor cells were determined. Moreover, estradiol （E2） and 1713-HSD1 mRNA levels were de- termined by ELISA and RT-PCR respectively. Results The tumor growth was inhibited in the genistein group and the anastrozole group. The inhibitory ratio was significantly higher in the genistein combined with anastrozole group （P 〈0.05）. Compared with the control group, levels of E2 and 1713-HSD1 mRNA decreased more significantly in the genistein combined with anastrozole group （P 〈0.05）. Conclusions Genistein could suppress the growth of mammary tumors in postmenopausal rats. It showed synergistic effect when combined with anastrozole, which resulted in reduced levels of E2 and 17β-HSD1 mRNA. It had inhibitory effect on the growth of breast tumors.

关 键 词：乳腺癌 染料木黄酮 阿那曲唑 绝经 

分 类 号：R737.9[医药卫生—肿瘤]