鱼藤酮对大鼠脑内多巴胺能神经元损伤的机制研究  

The damaged mechanism of rotenone on dopaminergic neuron of brain in rat

作　　者：吴艳芬[1] 王永春[2] 苏军红[3] 张延平[3] 王伟[3] 

机构地区：[1]邯郸市第一医院内分泌科,河北056001 [2]邯郸市中医院 [3]邯郸市第一医院神经内科

出　　处：《脑与神经疾病杂志》2014年第2期124-126,共3页Journal of Brain and Nervous Diseases

基　　金：河北省科技厅基金项目(132777134)

摘　　要：目的研究鱼藤酮对大鼠脑内多巴胺能神经元的毒性作用机制。方法健康成年雄性Wistar大鼠背部皮下注射鱼藤酮制备帕金森病动物模型。采用免疫细胞化学、透射电镜技术及分光光度法技术检测大鼠脑内多巴胺能神经元的损伤及纹状体中氧化应激参数的改变。结果鱼藤酮组大鼠中脑酪氨酸羟化酶(TH)免疫反应阳性神经元数明显少于对照组(P<0.01),纹状体中TH免疫反应强度明显降低(P<0.05),透射电镜可见鱼藤酮组黑质多巴胺能神经元内线粒体损伤,树突变性,其内微粒、微管聚集,同时纹状体发生了明显的氧化损伤。结论氧化应激和超微结构改变是鱼藤酮对大鼠脑内多巴胺能神经元损伤的主要发病机制。Objective The study of the toxic mechanism about rotenone on dopaminergic neurons of brain in＆nbsp;rats.Methods Healthy adult male Wistar rats were injected rotenone subcutaneous to prepare for Parkinson ＇s disease animal model.The damage of dopaminergic neurons of substantia nigra and striatum in rats brain was detected using immunocytochemistry , transmission electron microscopy and spectrophotometry .Results The TH immune response positive neuron numbers were significantly less in the midbrain of rotenone group as compared with control group （ P 〈0.01） .The TH strength of immune response in striatum significantly decreased in rotenone group ras （ P 〈0.05 ） .In the experimental group The microfilament microtubules gathered in the midbrain dopaminergic neurons, and oxidative damage significantly increased in striatum of rotenone group rats .Conclusions Oxidative stress and ultrastructure changes are the main pathogenesis of rotenone on dopaminergic neurons in rats .

关 键 词：鱼藤酮 帕金森病 多巴胺 氧化应激 透射电镜 

分 类 号：R742.5[医药卫生—神经病学与精神病学]