miR-455-3p在胃癌中的表达及其与MVD和VEGF表达的相关性研究  

Expression of miR-455-3p and its relationship with MVD and VEGF in gastric carcinoma

作　　者：宋鉴清[1] 曹伟[1] 吕昊玥 

机构地区：[1]中国医科大学附属第一医院检验科,沈阳110001

出　　处：《临床肿瘤学杂志》2014年第3期208-211,共4页Chinese Clinical Oncology

基　　金：辽宁省自然科学基金资助项目(201102291)

摘　　要：目的探讨miR-455-3p在胃癌中的水平及其与微血管密度(MVD)和血管内皮生长因子(VEGF)表达的关系。方法采用实时荧光定量PCR检测80例胃癌组织(胃癌组)中miR-455-3p水平,免疫组化法检测80例胃癌组织中MVD和VEGF表达,并分析胃癌组织中miR-455-3p水平与MVD和VEGF表达的相关性。选取同期的37例浅表性胃炎和12例正常胃黏膜组织作对照(对照组)。结果胃癌组的miR-455-3p水平为1.16±0.59,低于对照组的2.61±0.88,差异有统计学意义(P<0.05);胃癌组的VEGF阳性表达率和MVD分别为71.3%(57/80)和54.9±7.3,均高于对照组的28.6%(14/49)和27.5±6.1,差异均有统计学意义(P<0.05);胃癌组织中miR-455-3p水平与VEGF表达(r=-0.783,P<0.05)和MVD(r=-0.824,P<0.05)均呈负相关。结论 miR-455-3p在胃癌中为低表达,并与VEGF表达和MVD呈负相关,有可能在调控胃癌组织血管生长中发挥重要作用。Objective To investigate the level of miR-455-3p in gastric carcinoma and its relationship with microvascular density （ MVD） and vascular endothelial growth factor （ VEGF） . Methods The quantitative real-time PCR was used to examine the level of miR-455-3p in 80 gastric carcinoma tissues （ gastric carcinoma group） . The vascular endothelial growth factor （ VEGF） expres-sion and microvascular density （ MVD ） were measured by immunohistochemical method in 80 gastric carcinoma tissues. The correlations between level of miR-455-3p and MVD, VEGF expression were analyzed. Thirty-seven superficial gastritis and 12 normal gastric mucosa tissues were chosen as control （ control group） . Results The level of miR-455-3p in gastric carcinoma group was 1.16 ＆#177;0.59, lower than 2.61＆#177;0.88 in control group with significant difference （P＆lt;0.05）. The gastric carcinoma group.s positive expression rates of VEGF and MVD were 71.3% （57/80） and 54.9＆#177;7.3, higher than 28.6% （14/49） and 27.5＆#177;6.1 in control group with sig-nificant difference （ P＆lt;0.05） . The miR-455-3p was negatively correlated with VEGF expression （ r=-0.783, P＆lt;0.05） and MVD （ r=-0.824, P＆lt;0.05） in gastric carcinoma. Conclusion There was a decreased level of miR-455-3p in gastric carcinoma. The miR-455-3p was negatively correlated with VEGF expression and MVD in gastric carcinoma, which may play an important role in the regula-tion of blood vessel growth.

关 键 词：胃癌 微血管密度 血管内皮生长因子 

分 类 号：R735.2[医药卫生—肿瘤]