皮肤恶性肿瘤组织中B7-H1的表达及临床意义  

Expressions and clinical significance of B7-H1 in malignant cutaneous tumors

作　　者：郑云燕[1] 陈陆俊[2] 徐斌[2] 蒋敬庭[2] 吴昌平[2] 

机构地区：[1]苏州大学附属第三医院皮肤科,江苏常州213003 [2]苏州大学附属第三医院肿瘤生物诊疗中心,213003

出　　处：《临床肿瘤学杂志》2014年第3期221-225,共5页Chinese Clinical Oncology

基　　金：国家自然科学基金资助项目(30972703;81171653);江苏省卫生厅医学科技发展基金资助项目(P200932;P200933);常州市社会发展计划基金资助项目(CS20092019;S20102020);六大人才高峰第六批资金资助项目(BRA2010037)

摘　　要：目的研究共刺激分子B7-H1在常见皮肤恶性肿瘤组织中的表达及其临床意义。方法采用ElivsionTMplus免疫组织化学法检测B7-H1在51例鳞状细胞癌(SCC组)和93例非鳞状细胞癌共144例皮肤恶性肿瘤组织中的表达情况,同时选取10例日光性角化病(癌前病变组)和10例皮肤良性肿瘤(良性肿瘤组)作对照,分析B7-H1在癌前病变组、良性肿瘤组、SCC组及非鳞状细胞癌中的阳性表达率以及SCC中B7-H1表达水平与临床病理参数(年龄、性别、肿瘤大小、肿瘤部位、曝光部位及分化程度)间的关系。结果 B7-H1主要表达于皮肤肿瘤细胞的胞质及胞膜,呈弥漫性棕黄色颗粒状染色。B7-H1在癌前病变组、良性肿瘤组及SCC组的阳性表达率分别为10.0%(1/10)、40.0%(4/10)和76.5%(39/51);在SCC中,B7-H1表达水平在不同年龄、性别、肿瘤大小、肿瘤部位、曝光部位及分化程度间的差异均无统计学意义(P>0.05)。B7-H1在常见上皮来源的非鳞状细胞癌中均有表达,其中基底细胞癌、汗腺癌、乳房外Paget病、腺样囊性癌、皮脂腺癌及恶性黑色素瘤的阳性表达率依次为81.4%(35/43)、70.0%(7/10)、100.0%(3/3)、100.0%(2/2)、100.0%(1/1)和76.2%(16/21)。而在非上皮来源的隆突性纤维肉瘤中未见表达。结论皮肤SCC组织中B7-H1表达率高于癌前病变和皮肤良性肿瘤组织,提示B7-H1可能参与了皮肤恶性肿瘤的发生与发展。Objective To explore the expressions of costimulatory molecule B7-H1 in malignant cutaneous tumor tissues and their clinical significance. Methods The expressions of B7-H1 were detected by ElivsionTM plus immunohistochemical technique in tumor tissues from 51 patients with squamous cell carcinoma （ SCC group） and 93 patients with non-squamous cell carcinoma. Tissues obtained from 10 patients with actinic keratosis （ precancerous lesions group） and 10 patients with benign cutaneous tumors （ benign tumor group） were chosen as control. The positive rates of B7-H1 in precancerous lesions group, benign tumor group, SCC group and non-squamous cell carcinoma were analyzed. The relationships between expressions of B7-H1 and clinicopathological characteristics （ age, sex, tumor size, tumor location, exposure, location and degree of differentiation） in SCC were investigated. Results The B7-H1 was characterized by diffuse brown staining and mainly expressed in cell membrane and cytoplasm. The positive rates of B7-H1 in precancerous lesions group, benign tumor group and SCC group were 10.0% （ 1/10） , 40.0% （ 4/10） and 76.5% （ 39/51） , respec-tively. There was no significant correlation between expression of B7-H1 and clinicopathological characteristics. The B7-H1 was also ex-pressed in common epithelial origin non-squamous cell carcinoma, including basal cell carcinoma, sweat gland carcinoma, Paget.s dis-ease, adenoid cystic carcinoma and sebaceous carcinoma with positive expression rates of 81.4% （ 35/43） , 70.0% （ 7/10） , 100.0%（ 3/3） , 100.0% （ 2/2） , 100.0% （ 1/1） and 76.2% （ 16/21） . No expression was observed in the non-epithelial origin bulge fibrosar-coma. Conclusion B7-H1 is involved in the carcinogenesis and development of malignant cutaneous tumor.

关 键 词：B7-H1 皮肤恶性肿瘤 免疫组织化学 共刺激分子 

分 类 号：R739.5[医药卫生—肿瘤]