Reagent-free determination of amikacin content in amikacin sulfate injections by FTIR derivative spectroscopy in a continuous flow system  

作　　者：José F.Ovalles Máximo Gallignani María R.Brunetto Rebeca A.Rondón Carlos Ayala 

机构地区：[1]Departamento de Análisis y Control,Facultad de Farmacia y Bioanálisis,Universidad de Los Andes,Mérida 5101A,Venezuela [2]Laboratorio de Espectroscopia Molecular,Departamento de Química,Facultad de Ciencias,Universidad de Los Andes,Mérida 5101A,Venezuela

出　　处：《Journal of Pharmaceutical Analysis》2014年第2期125-131,共7页药物分析学报（英文版）

基　　金：the CDCHTA of the University of Los Andes for providing financial support through several approved projects;the National Fund for Science, Technology and Innovation (FONACIT) of Venezuelan Ministry of Science and Technology for providing financial support, SPE 112–370 and Project G-2005000641

摘　　要：The quantitative estimation of amikacin （AMK） in AMK sulfate injection samples is reported using FTIR-derivative spectrometric method in a continuous flow system. Fourier transform of mid-IR spectra were recorded without any sample pretreatment. A good linear calibration （r40.999, %RSDo 2.0） in the range of 7.7-77.0 mg/mL was found. The results showed a good correlation with the manufacturer＇s and overall they all fell within acceptable limits of most pharmacopoeial monographs on AMK sulfate.The quantitative estimation of amikacin （AMK） in AMK sulfate injection samples is reported using FTIR-derivative spectrometric method in a continuous flow system. Fourier transform of mid-IR spectra were recorded without any sample pretreatment. A good linear calibration （r40.999, %RSDo 2.0） in the range of 7.7-77.0 mg/mL was found. The results showed a good correlation with the manufacturer＇s and overall they all fell within acceptable limits of most pharmacopoeial monographs on AMK sulfate.

关 键 词：AMIKACIN FlrIR derivative spectro-metry Continuous flow system Pharmaceuticalpreparation INJECTION SULFATE 

分 类 号：R927.2[医药卫生—药学]