17β-雌二醇对小鼠子宫DLL1基因表达的影响  

Effects of 17β-estrogen on gene DLL1 expression in murine uterine tissue

作　　者：马法洋[1] 林爱琴[1] 李铁臣[1] 

机构地区：[1]皖南医学院分子生物学研究室,安徽芜湖241002

出　　处：《皖南医学院学报》2014年第2期108-110,共3页Journal of Wannan Medical College

摘　　要：目的:研究高水平17β-雌二醇持续刺激去卵巢小鼠,对子宫组织DLL1基因表达的影响。方法:将16只小鼠随机平均分为正常对照组和去卵巢E2实验组(手术造模+E2干预);TRIzol一步法提取两组小鼠子宫组织总RNA,采用RT-PCR法扩增目的基因及内参基因的目的片段,紫外光下观察电泳结果并采用QuantityOne-4.6.2凝胶分析软件测定目标带光强度值。结果:两组小鼠子宫组织标本均出现目的基因DLL1与内参基因Gapdh的目标带,DLL1/Gapdh光强度的比值分别为0.33±0.12、0.78±0.11,两者差异有统计学意义(P<0.05),并且去卵巢E2实验组明显高于正常对照组。结论:高剂量E2的持续干预可显著提高去卵巢小鼠子宫组织DLL1基因的表达水平,可能是雌激素依赖性子宫组织细胞不正常增殖的病理基础。Objective：To examine the effects of constant high-dose 17β-estrogen stimulation on the Delta-like protein 1（DLL1） level in the uterine tissue of ovariectomized mice.Methods：Sixteen mice were randomly divided into normal control group and ovariectomized E2 experimental group （Model ＋E2 intervention）.TRIzol reagent was used to extract the total RNA from the uterine tissues,and reverse transcription polymerase chain reaction（RT-PCR） was used to amplify the target sequences and internal control gene that were observed under UV light measured for the value of Adj .Vol with QuantityOne-4.6.2.Results：The target bands of gene DLL1 and Gapdh were detected in all samples,and the DLL1/Gapdh Adj.Vol.value of the two groups were 0.33 ＆#177;0.12,0.78 ＆#177;0.11,respectively.The two groups were statistically different（P ＆lt;0.05）,and the expression level was significantly higher in E2 group than the normal control group.Conclusion：Lasting high-dose estrogen stimulation could increase the gene DLL1expression in the uterine tissue of mice,which may explain the pathomechanism for abnormal growing of uterine tissue resulted from estrogen dependence .

关 键 词：17Β-雌二醇 子宫组织 DLL1基因 

分 类 号：R394.3[医药卫生—医学遗传学]