螺内酯诱导人急性白血病细胞Jurkat凋亡的作用研究  

Study on apoptosis of human T-acute lymphoblastic leukemia cells lines Jurkat treated with spironolactone

作　　者：韩莲花[1] 蔡磊[1] 朱莹[1] 朱华亭[1] 夏永洁[1] 邱玉华[1] 

机构地区：[1]苏州大学医学部免疫学系,苏州215123

出　　处：《实用药物与临床》2014年第4期399-401,共3页Practical Pharmacy and Clinical Remedies

基　　金：江苏省普通高校研究生科研创新计划(CXLX12_0821)

摘　　要：目的研究螺内酯对人急性白血病细胞Jurkat体外增殖的抑制及诱导凋亡的作用。方法将终浓度分别是10、50及100μmol/L的螺内酯加入Jurkat细胞的培养体系中,24 h内每隔4 h通过MTT法分析Jurkat细胞的增殖抑制率,Annexin V/PI流式细胞术法分析Jurkat细胞的早期凋亡率。结果螺内酯与Jurkat细胞共同培养12 h后,螺内酯能显著增加对细胞的增殖抑制作用,并且与药物浓度成正相关,各浓度组的抑制率随着培养时间的延长而升高,与药物干预时间成正相关;螺内酯处理Jurkat细胞24 h,各浓度组诱导细胞凋亡率分别为:10μmol/L组(11.2±0.35)%、50μmol/L组(29.8±1.27)%及100μmol/L(56.5±1.41)%,各个浓度组诱导细胞凋亡率与药物浓度成正相关。结论螺内酯对人T淋巴细胞白血病Jurkat细胞具有抑制增殖和诱导凋亡的作用,提示该药可能具有潜在抑瘤作用。Objective To study the proliferated inhibition and apoptosis of human T-acute lymphoblastic leu-kemia cells lines Jurkat treated with spironolactone. Methods Jurkat cells were treated with different concentrations of spironolactone （10,50,100 μmol/L） in culture. MTT assay was used to observe the proliferated inhibition rate of Jur-kat at 4 hours interval and the flow cytometry was applied to analyze Annexin V/PI apoptosis rate of Jurkat. Results 12 hours after treatment,spironolactone could obviously inhibit the proliferation of Jurkat and the effect had dose and treated time dependence. 24 hours after treatment, apoptosis of these concentrations were （ 11. 2 ＆#177; 0. 35 ）%, （ 29. 8 ＆#177; 1. 27）%,（56. 5 ＆#177; 1. 41）%,and the apoptosis rate increased in dose dependence. Conclusion Spironolactone can in-hibit the proliferation and induce apoptosis of Jurkat,it suggests that this medicine may have the effect of tumor inhibi-tion.

关 键 词：螺内酯 增殖抑制 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]