抗阻训练与耐力训练对小鼠腓肠肌卫星细胞激活作用的影响  

Effect of Resistance and Endurance Training on the Activation of Muscle Satellite Cells in the Gastrocnemius of Mice

作　　者：陈彩珍[1,2] 卢健[1,2] 

机构地区：[1]青少年健康评价与运动干预教育部重点实验室华东师范大学,上海200241 [2]华东师范大学体育与健康学院,上海200241

出　　处：《成都体育学院学报》2014年第4期55-59,72,共6页Journal of Chengdu Sport University

基　　金：上海市浦江人才计划资助项目(项目编号:10PJC029)

摘　　要：通过观察骨骼肌肥大相关细胞因子mRNA的表达,探讨抗阻训练或耐力训练对骨骼肌卫星细胞增殖分化能力的作用及可能机制。选取21只雄性老年SAMP8小鼠,随机分为对照组(C),耐力训练组(E,8周跑台训练),抗阻训练组(R,8周尾部负重爬梯训练)。经过8周的训练后,检测小鼠右侧腓肠肌横截面积及IGF-1、MGF、MyoD及myogenin等mRNA的表达。结果发现耐力训练和抗阻训练大鼠腓肠肌IGF-1、MGF、MyoD及myogenin等mRNA的表达均显著高于对照组;两训练组间比较,抗阻训练组MGFmRNA显著高于耐力训练组(P<0.01),其它3个基因的mRNA有上调趋势,但无显著性差异;抗阻训练组的腓肠肌纤维横截面积显著高于对照组和耐力训练组。抗阻训练在维持肌质量方面效果优于耐力训练,推测这两种不同的运动方式对腓肠肌质量影响之差异可能与MGF介导的骨骼肌卫星细胞的激活有关。Objective ： To observe the effect of endurance training or resistance training on the expression of IGF - 1 ,MGF,MyoD and myogenin mRNA in gastrocnemius muscle, and to explore its role in the activation of muscle satellite cells. Methods： 21 SAMP8 senile male mice were randomly distributed into three groups： sedentary （CON, n =7） , endurance training group （n = 7）, resistance training groups （n = 7）. After 8wk training, gastroenemius muscles were excised and muscle - wet weight and body mass （BM） are weighed and analyzed by real - time PCR. Results ： The ratio of muscle wet weight and BM （RM） of resistance training group showed significant differences when compared with that of the endurance group or control group ,8wk endurance training or resistance training resulted in an increase in mRNA levels of IGF - 1, MGF, MyoD and myogenin, whereas two training groups have no significant difference except IGF - 1 mRNA which were significantly higher in resistance training group than those in endurance group. Conclusion： Resist- ance training is better than endurance training in maintaining muscle quality effect. Two different exercise ways might be through the MGF which represent potential mediators implicated in the regulation of hypertrophy during exercise to acti- vate the muscle satellite cells.

关 键 词：骨骼肌肥大 IGF-1 生肌调节因子 耐力训练 抗阻训练 

分 类 号：G804.2[文化科学—运动人体科学]