高效液相色谱法测定烤烟非挥发性有机酸含量  

Content determination for nonvolatile organic acids in tobacco by HPLC

作　　者：尹莉丽[1] 赵百东[2] 杨虹琦[1] 柳立[1] 陈丽娟[1] 费明亮[1] 

机构地区：[1]湖南农业大学烟草科学与健康重点实验室,湖南长沙410128 [2]国家烟草专卖局,北京100053

出　　处：《湖南农业大学学报（自然科学版）》2014年第2期139-143,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家烟草专卖局项目(11–14Aa01;2013NK3073)

摘　　要：采用高效液相色谱技术（HPLc）建立了一种同时快速测定烟叶中草酸、苹果酸、乳酸、柠檬酸、琥珀酸等非挥发性有机酸的方法。采用磷酸溶液，80℃水浴30min，提取烟叶样品中的非挥发性有机酸，回收率可达到85．37％以上；采用Ultimate，AQ-C18反相色谱柱，在215nm波长下，以0．02mol／LKH2PO4溶液（pH3．0）和甲醇作为流动相，可较好地分离和测定初烤烟叶中的非挥发性有机酸含量，相对标准偏差为0．58％～1．99％，最低检出限为0．03～0．80μg／mL。A method of reverse-phase high performance liquid chromatography（HPLC） for simultaneous and rapid determination of oxlate, malic, lactic, citric and succinic acid was established. The results showed that the recoveries of nonvolatile organic acid reached above 85.37% by phosphorous acid extracting under a water bath at 80 ℃ for 30 min. Using Welch Ultimate, AQ-C18 column with KHEPO4 （0.02 mol/L, pH3.0） and methyl alcohol as mobile phase at the detection wavelength of 215 nm, the nonvolatile organic acid in tobacco were well separated and their contents were well determined with relative standard deviations being 0.58% -1.99% and detection limits being 0.03-0.80 μg/mL.

关 键 词：烤烟 高效液相色谱法 非挥发性有机酸 

分 类 号：S572.01[农业科学—烟草工业]