木耳总RNA提取方法研究  

Study on methods for RNA extraction from Auricularia auricular

作　　者：熊毅[1] 郭彦萃[1] 张志[1] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《浙江农业学报》2014年第2期456-460,共5页Acta Agriculturae Zhejiangensis

基　　金：黑龙江省教育厅科学技术研究项目(1551538)

摘　　要：为了获得木耳总RNA理想的提取方法,为后续的分子生物学研究打下基础,以木耳菌丝为试验材料,采用了CTAB法、RNA prep pure Plant Kit试剂盒和Trizol法3种方法提取木耳总RNA。结果表明3种方法均能从木耳菌丝中提取分离到总RNA,然而Trizol法能提取到纯度较高、完整性较好的总RNA,28S及18S带型清晰无弥散,A260/A280值为1.91,RNA产率为682.64μg·g-1,提取的总RNA适用于RT-PCR等分子生物学试验研究。The purity and integrity of total RNA are essential for molecular biology experiments of Auricularia auricular.In order to obtain an ideal total RNA extraction method , total RNA of Auricularia auricular was extracted with 3 methods, including Trizol method, CTAB method and RNA prep pure Plant Kit method .The results showed that all these methods can extract total RNA fromA ur icularia auricular;however, the Trizol method was the best with high integrity and purity.The band of 28S was twice wider than that of 18S.The RNA yield was 682.64 μg·g-1 and the RNA sample could be used for RT-PCR.

关 键 词：木耳 RNA提取 TRIZOL法 

分 类 号：S646.6[农业科学—蔬菜学]