子宫颈鳞状细胞癌纤维结合素、层黏蛋白及Ⅳ型胶原的异常表达  

Expression of Fibronectin,Laminin and Type Ⅳ Collagen in Cervical Carcinoma

作　　者：娄阁[1] 钱素娟[2] 高庆玉[1] 宁小明[1] 隋丽华[1] 马宝璋[2] 

机构地区：[1]哈尔滨医科大学第三临床医院,150040 [2]哈尔滨医科大学

出　　处：《诊断病理学杂志》2001年第1期35-37,T015,共4页Chinese Journal of Diagnostic Pathology

基　　金：黑龙江省自然科学基金资助课题!(D972 8)

摘　　要：目的　通过对纤维结合素 (Fibronectin ,FN)和层黏连蛋白 (Laminin ,LN)及Ⅳ型胶原蛋白的表达进行检测 ,探讨基底膜重构与宫颈癌分化程度的关系。方法　应用双重免疫荧光染色技术 ,对正常人及不同分化程度的子宫颈鳞状细胞癌病人子宫颈石蜡包埋组织的FN、LN及Ⅳ型胶原表达进行检测 ,并应用共聚焦显微镜观察结果。结果　正常子宫颈组织中 ,细胞外间质内Ⅳ型胶原和LN蛋白呈线状环绕于血管及上皮下基底膜内 ,FN呈细条索分布于基底膜和间质内。子宫颈癌组织中 ,癌细胞团周围基底膜内的Ⅳ型胶原的线状荧光减弱、断裂甚至缺失 ,尤其以低分化鳞状细胞癌明显。而FN和LN蛋白表达增多 ,并与癌细胞分化程度负相关。一些癌细胞浆内可见FN和LN蛋白的阳性染色。结论　子宫颈鳞状细胞癌时 ,基底膜变薄、断裂 ,Ⅳ型胶原降解。而癌细胞浆及邻近癌组织的间质内 ,FN及LN表达增多 。Objective To explore the relationship between remodeling basement membrane and differentiation of cervical squamous cell carcinoma. Methods Dual fluorescent labeling technique was used to demonstrating the expression of fibronectin(FN),laminin(LN) and type Ⅳ collagen in formalin fixed,paraffin embedded tissue of cervical normal and squamous cell carcinoma,and the results were detected by a confocal laser scanning microscopy. Results The subepithelial and vessel basement membrane staining of FN,LN and collagen Ⅳ revealed positive in normal cervical tissue.In the cervical squamous cell carcinoma,the staining for collagen Ⅳ showed discontinuity,fragmentation or absence of basement membtane in the vicinity of cervical carcinoma,but the expression of LN and FN increased,particularly in low grade invasive carcinomas. Conclusions In the cervical squamous cell carcinoma,Ⅳ collagen is degraded,but expression of LN and FN increases.The basement membrane has been remodeled,which is correlated to grades of differentiation of the tumor.

关 键 词：子宫颈鳞状细胞癌 纤维结合素 层黏蛋白 Ⅳ型胶原 

分 类 号：R737.33[医药卫生—肿瘤]