脱毒马铃薯六种酶的活性变化分析  

Changes of Activity of Six Enzymes in Virus-free Potato Seedlings

作　　者：刘志文[1] 闫明明[1] 郭晶[1] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《吉林农业科学》2014年第2期71-73,共3页Journal of　Jilin Agricultural Sciences

基　　金：大连市科学技术基金项目(2010J21DW015)

摘　　要：本文对马铃薯脱毒苗和经优化培养后的脱毒苗在不同时期的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)、果胶酶和纤维素酶6种酶的活性变化进行分析。结果表明:脱毒苗和优化培养的脱毒苗表现出相同的酶活变化趋势,不同时期4种保护性酶活性均较其对照提高,而果胶酶和纤维素酶的活性反而降低,这将会提高它们的抗性,为获得高产提供了基础。Dynamic changes in activities of superoxide dismutase （SOD）, peroxidase （POD）, catalase （CAT）, polyphenoloxidase （PPO）, pectinase and cellulase of the virus-free potato seedlings and plantlets cul- tured in the optimal condition were measured and analyzed during different growth period in this study. The results showed that the virus-free and the optimal cultured seedlings displayed the same change trends, the activities of SOD, POD, PPO, CAT increased significantly and the activities of pectinase and cellulose de- creased compared with the control. These changes could increase resistance of the potato seedlings and pro- vide basis for their high-yield.

关 键 词：马铃薯 脱毒 酶活 变化分析 

分 类 号：S532.01[农业科学—作物学]