双酚A对MCF-7细胞内DNA甲基化的影响  

Changes Of genome DNA methylation induced by bisphenol A in MCF-7 cells

作　　者：刘翅琼[1] 赵琳[1] 陈锋[1] 

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001

出　　处：《实用预防医学》2014年第4期416-419,共4页Practical Preventive Medicine

摘　　要：目的探讨双酚A对MCF-7细胞内整体DNA甲基化水平及甲基化转移酶的影响。方法分别将浓度为10-6mol/L、10-7mol/L的双酚A作用于MCF-7细胞72 h后,免疫荧光法测5-甲基胞嘧啶的含量,RT-PCR法测DNMT1、DNMT3A、DNMT3B mRNA的表达。结果双酚A(10-6mol/L、10-7mol/L)作用72 h后,与溶剂对照组(0.1%DMSO)相比,5-甲基胞嘧啶的含量下降,差异有统计学意义(P<0.05);RT-PCR结果表明浓度为10-6mol/L双酚A处理组的DNMT1的mRNA的表达下降,10-6mol/L、10-7mol/L双酚A组的DNMT3A和DNMT3B的mRNA的表达均增加,差异有统计学意义(P<0.05)。结论双酚A能够引起MCF-7细胞整体DNA甲基化水平下调,其机制可能是通过改变甲基化转移酶的表达来实现。Objective To investigate the effect of bisphenol A （BPA） on the general level of DNA methylation and the expression of DNA methyltransferases （DNMTs） in MCF-7 cells.Methods MCF-7 cells were respectively treated with 10-6 mol/L and 10-7 mol/L BPA for 72 hours.The DNA methylation levels were determined by 5-methylcytosine immunoflurescence assay.The mRNA expression of DNMT1,DNMT3A and DNMT3B were measured by RT-PCR.Results Compared with the control group （0.1% DMSO）,the amounts of 5-methylcytosine in the experimental groups decreased significantly （P 〈0.05）.The mRNA expression of DNMT1 decreased in 10-6 mol/L BPA group,while those of DNMT3A and DNMT3B significantly increased in 10-6 mol/L and 10-7 mol/L BPA groups （P 〈 0.05）.Conclusions BPA can reduce the methylation level in MCF-7 cells,and the mechanism of which may be associated with the changes of DNMTs.

关 键 词：双酚A DNA甲基化 DNMT1 DNMT3A DNMT3B 

分 类 号：R-332[医药卫生]