深海适冷菌SM9913产生的低温蛋白酶  

PSYCHROTROPHILIC ALKALINE PROTEASE FROM A DEEP SEA PSYCHROTROPHILIC Strain Pseudomonas sp. SM9913

作　　者：陈秀兰[1] 张玉忠[1] 王运涛[1] 高培基[1] 栾裼武[2] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100 [2]中国科学院海洋研究所,青岛266071

出　　处：《海洋科学》2001年第1期4-8,共5页Marine Sciences

基　　金：863计划海洋生物技术主题资助项目!819 02 02号

摘　　要：从1855m深的深海泥样中分离纯化得到200多株分泌蛋白酶的适冷菌 ,其中3株产低温蛋白酶 ,本文对其中一株Pseudomonassp.SM9913(P.SM9913)生长的适冷性和它产生的蛋白酶的适低温特性进行了研究。该菌株能够在0℃下正常生长 ,其最适生长温度为15℃ ,最高生长温度为35℃。为一株适冷菌。该菌株所产蛋白酶的比合成速率在10℃时最高 ,催化酪蛋白水解的最适温度为35℃ ,在0℃仍具有3 %的酶活力。最适 pH为8.0。该蛋白酶的热稳定性很低 ,在40℃保温10min即丧失85 %的活力 ,40℃时的半衰期为6min,为一典型的低温酶。抑制剂试验表明 。More than 200 psychrotrophilic strains excreting protease were isolated from 1 855 meter deep sea silt and were puri-fied. 3 strains of them excreted psychrotrophilic protease. The psychrotrophilic characteristics of the protease excreted by one of the three strains——Pseudomonas sp. SM9913(P.SM9913)were preliminarily studied. Strain P. SM9913 could grow at 0 ℃. The optimum temperature for growth was about 15 ℃. And the highest temperature for growth was 35 ℃, which suggested that stain P. SM9913 was psychrotrophilic. The highest specific synthetic rate of the protease occurred at about 10 ℃. The optimum temperature of the protease for casin catalysis was 35 ℃, and the highest protease activity for casin catalysis was at pH8.0. The protease was susceptible to heat treatment, and the half time for the decrease of protease activity was 6 minutes when incubated at 40 ℃. These results showed that this protease was psychrotrophilic. The activity of the protease was inhibited by EDTA, indicating that this protease belonged to metalprotease.

关 键 词：深海 适冷菌 低温蛋白酶 温度 产酶条件 

分 类 号：Q938.8[生物学—微生物学] Q936