pcDNA3.1表达载体转染对细胞生长的影响  

Effects of pcDNA3. 1 expression vector transfection on cell growth

作　　者：余鹰[1] 周鸣[1] 曹利[1] 唐珂[1] 李伟芳[1] 李桂源[1] 

机构地区：[1]湖南医科大学肿瘤研究所,湖南长沙410078

出　　处：《生物技术》2001年第1期1-3,共3页Biotechnology

基　　金：国家高科技"863"项目资助(102-10-01-05);国家"973"重点项目资助;国家自然科学基金项目资助(397001

摘　　要：为了探讨真核表达载体转染对细胞生长的影响，通过脂质体介导将ｐｃＤＮＡ３．１（＋）表达载体ＤＮＡ转染鼻咽癌细胞系 ＨＮＥ１，Ｇ４１８筛选后，Ｓｏｕｔｈｅｒｎ杂交鉴定稳定表达细胞株，以 ＨＮＥ１细胞为对照，观察ｐｃＮＡ３．１（＋）／ＨＮＥ１克隆细胞的生物学特性；结果显示，在ｐｃＤＮＡ３．１（＋）／ＨＮＥ１阳性克隆中，一株细胞克隆培养过程中发生自溶性死亡，一株细胞生长明显受到抑制，另一株细胞生长无明显影响，揭示在宿主细胞中ｐｃＤＮＡ３.１（＋）ＤＮＡ与宿主基因组ＤＮＡ发生了随机整合，从而表现不同的细胞生物学改变。pcDNA3 .1 expression vector is very popular for researcher to study gene function. In this paper, pcDNA3 .1 ( +) expression vector has been used to investigate its effects on HNE1 cell line by lipid transfection, the cytobiology of pcDNA3. 1 ( + )/HNE1 cell line were analysised. Among the pcDNA3. 1 (-)/HNE1 positive clones detected, one was died autolytically in the process of cell culture; one grew slower than HNE1 ,the other had no evident change. This results implied pcDNA3. 1 ( +) plasmid DNA had integrated randomly with the chromosomes of host cell, and different biological effects appeared.

关 键 词：真核表达系统 PCDNA3.1(+)载体 整合 哺乳动物细胞 

分 类 号：Q78[生物学—分子生物学]