非小细胞肺癌化疗相关基因标志物表达特征分析  

Expression characteristics of chemotherapeutic related genes in non-small cell lung cancer

作　　者：张诗珩[1] 李峥[1] 彭宇[1] 彭杨[1] 杨宇馨[1] 李崇义[1] 肖何[1] 王东[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所肿瘤中心,重庆400042

出　　处：《重庆医学》2014年第11期1284-1286,1289,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(81171904)

摘　　要：目的探讨非小细胞肺癌(NSCLC)化疗药物相关基因标志物的表达谱特征及其与病理类型的关系,为临床个性化化疗方案提供实验依据。方法采用免疫组织化学分析733例NSCLC患者的剪切修复交叉互补基因1(ERCC1)、乳腺癌易感基因(BRCA1)、脱嘌呤/脱嘧啶核酸内切酶1(APE1)、核糖核苷还原酶M1(RRM1)、β-TubulinⅢ家族(TUBB3)和胸苷酸合成酶(TS)6种化疗药物相关标志物的表达特征,分析6种标志物与病理的关系及其相互间的相关性。结果 BRCA1、TUBB3、TS表达在腺癌、鳞癌、未分类癌3种病理类型间比较,差异有统计学意义(P<0.01);且这3种标志物表达与病理类型有关(r=0.107、r=-0.229、r=0.168,P<0.01)。ERCC1与BRCA1、ERCC1与APE1、APE1与RRM1之间呈正相关(r=0.214、r=0.316、r=0.222,P<0.01)。结论 NSCLC化疗相关标志物表达与临床病理类型有关,多种标志物表达相互间具有显著相关性。对化疗药物相关标志物表达及病理类型进行联合检测有助于临床制订个体化化疗方案。Objective To analyze the expression characteristics of chemotherapeutic related genes （ERCC1, BRCA1, APE1, RRM1 ,TUBB3 and TS） in non-small cell lung cancer（NSCLC） and their associations with the pathological types to provide the ex- periElental evidences for clinical individual chemotherapy. Methods The immunohistochemical SP method was used to determine the expression of ERCC1,BRCA1, APE1, RRM1, TUBB3 and TS genes in 733 patients with NSCLC. Chi-square test was performed to analyze the gene expression characteristics among different pathological types. Spearman relationship was conducted to evaluate the associations among the six genes and different pathological types. Results The expression of BRCA1 ,TUBB3 and TS had sta- tistical differences among three pathological types（P〈0.01） sand these three genes showed a relationship with the pathological type （r：0. 107=0. 229 and 0. 168,respectively,P〈0.01）. ERCC1 vs. BRCA1,ERCC1 vs. APE1 and APE1 vs. RRM1 showed the pos- itive correlation（r=0. 214,0. 316 and 0. 222,respectively,P〈0.01）. Conclusion The chemotherapeutic related gene expressions of NSCLC are related with the clinical pathological types. And the expressions of multiple markers are associated with each other. The combined detection of the chemotherapeutic related markers and the pathological types would be helpful to the decision making of individual chemotherapy in clinic.

关 键 词：癌 非小细胞肺 抗肿瘤联合化疗方案 生物学标记 脱嘌呤 脱嘧啶核酸内切酶 

分 类 号：R734.2[医药卫生—肿瘤]