鸡白痢沙门氏菌O_(12)特异性噬菌体的分离及生物学特性研究  

Study on isolation and biological characterization of bacteriophage of salmonella O_(12) for pullorum

作　　者：李涵依[1] 车晨[1] 耿亚[1] 刘福音[1] 郝葆青[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041

出　　处：《西南民族大学学报（自然科学版）》2014年第2期186-191,共6页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：西南民族大学研究生创新项目

摘　　要：采用双层平板培养法从成都某养鸡场的污水样本中分离得到一株鸡白痢沙门氏菌O12(宿主菌)的噬菌体(Ph-1),并对菌斑形态及理化特性进行了初步研究.试验结果显示,纯化后该噬菌体的噬菌斑直径约3mm;经高于60℃温度处理1h后噬菌体失活;PH5-7时该噬菌体可保持较高效价和较强的紫外线耐受性;该噬菌体的最佳感染复数(MOI)为0.01-0.001;一步生长曲线显示该噬菌体的潜伏期为40min,裂解期持续60min.One bacteriophage, named Phi, was isolated from waste-water samples collected from a Chengdu hennery by the double-agar method, and was to be obligate on the salmonella O12 pullorum （Bacillary white diarrhea） that was as host bacteria. The biological catheterization was explored on this bacteriophage. The results indicated that the diameter of Phi phages was about 3ram. When incubated in 60℃ or higher for lh, the phage was inactivated. It was able to keep a high activity at a range of pHS-7. In the UV-test it was demonstrated that Phl possesses a strong ant-UV ability. The best MOx of Phi was 0.01-0.001. It showed that the latent phase and rise phase were 40min and 60 min respectively on the one-step frowth curve.

关 键 词：鸡白痢 噬菌体 沙门氏菌 

分 类 号：S852.61[农业科学—基础兽医学]