瘢痕中肌动蛋白及肌球蛋白的实验研究  

EXPERIMENTAL STUDY OF ACTIN AND MYOSIN IN THE SCAR TISSUE

作　　者：张群[1] 钱云良[1] 崔磊[1] 

机构地区：[1]上海第二医科大学附属第九人民医院整形外科,上海200011

出　　处：《中国修复重建外科杂志》2001年第1期32-35,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金!资助项目 (395 70 72 3)

摘　　要：目的　探讨增生性瘢痕、瘢痕疙瘩及正常皮肤成纤维细胞中肌动蛋白和肌球蛋白 的不同表达情况及其与瘢痕挛缩的关系。方法　取增生性瘢痕 15例 ,瘢痕疙瘩 10例 ,正常皮肤 15例 ,用免疫组织化学方法检测其中肌动蛋白和肌球蛋白 的表达情况 ,同时做细胞培养 ,用流式细胞术检测成纤维细胞中肌动蛋白和肌球蛋白 的表达情况。结果　增生性瘢痕肌球蛋白 的免疫组织化学染色呈阳性 ,而瘢痕疙瘩及正常皮肤肌球蛋白 的表达均为阴性。增生性瘢痕肌球蛋白 的表达较其它两种组织有非常显著性差异 (P<0 .0 1) ,而瘢痕疙瘩和正常皮肤无显著性差异 (P>0 .0 5 )。流式细胞术检测增生性瘢痕、瘢痕疙瘩及正常皮肤三种组织中肌球蛋白 的阳性率分别为 (95 .11± 2 .78) %、(16 .86± 7.11) %及 (5 .31± 1.79) % ,增生性瘢痕肌球蛋白 表达的阳性率较其它两种组织有非常显著性差异 (P<0 .0 1) ,而瘢痕疙瘩和正常皮肤无显著性差异 (P>0 .0 5 )。三种组织中肌动蛋白的免疫组织化学染色均为阳性 ,无显著性差异 (P>0 .0 5 )。流式细胞术检测三种组织中肌动蛋白的阳性率分别为 (77.77±15 .43) %、(88.89± 10 .2 9) %及 (82 .92± 13.48) % ,其表达的阳性率无显著性差异 (P>0 .0 5 )。结论　瘢痕挛缩与肌球蛋白Objective To investigate the different expression of actin, myosin Ⅱ in hypertrophic scars, keloids and normal skins, and to understand the relationship of actin, myosin Ⅱ and the scar contracture. Methods Fifteen cases with hypertrophic scars, 10 cases with keloids and 15 cases with normal skins were chosen randomly. The expression of actin and myosin Ⅱ were detected by immunohistochemical method in the hypertrophic scars, keloids and normal skins. The fibroblasts isolated from three types of tissue were cultured in vitro, then actin and myosin Ⅱ in three different fibroblasts were measured using flow cytometry. Results The immunohistochemical staining of myosin Ⅱ in hypertrophic scars was positive, while the staining in keloids and normal skins were negative. The positive rate of myosin Ⅱ expression in hypertrophic scars, keloids and normal skins were (95.11±2 78)%, (16.86±7.11)%, and (5.31±1.79)% respectively. There were significant difference between keloids and the two others( P <0.01). The actin expression in three difference tissues were positive, there were no significant difference in hypertrophic scars, keloids and normal skins( P >0.05). The positive rate of actin expression in hypertrophic scars, keoids and normal skins were(77.77±15.43)%, (88.89±10.29)%, and (82.92± 13.48)% respectively, and there were no significant difference( P >0.05). Conclusion Myosin Ⅱ may play an important role in the scar contracture. Actin is the contractile protein of cell, it plays important role in cellular movement. Actin is necessary protein in the cell.

关 键 词：成纤维细胞 肌动蛋白 肌球蛋白 瘢痕挛缩 

分 类 号：R363.1[医药卫生—病理学]