小动物活体生物发光成像技术在肿瘤转移研究中的应用  

Application of in vivo bioluminescence imaging on the study of tumour metastasis

作　　者：范临兰[1] 李楠[2] 席晓霞[1] 罗旭飞 李娜[2] 张建刚[1] 魏虎来[1] 

机构地区：[1]兰州大学基础医学院甘肃省新药临床前研究重点实验室,甘肃兰州730000 [2]兰州大学

出　　处：《实验室科学》2014年第2期12-16,19,共6页Laboratory Science

基　　金：甘肃省中医药科学技术研究课题(GZK-2010-17);兰州大学大学生创新创业项目(项目编号:201310730158)

摘　　要：建立小动物活体生物发光成像技术,研究三氧化二砷(As2O3)抑制小鼠B16恶性黑色素瘤的肺转移作用的方法,比较活体生物发光成像技术与常规动物实验技术的优劣性;并探讨As2O3抗小鼠恶性黑色素瘤肺转移的可能机制。结果表明,活体生物发光成像技术可以非侵袭性地动态监测黑色素瘤B16细胞在小鼠肺部的转移过程以及评估As2O3的体内抑制B16肺转移的作用,且活体生物发光成像技术的检测灵敏度优于传统肺转移瘤结节计数技术;As2O3主要通过减低肿瘤新生血管形成、促使肿瘤组织坏死而发挥抗小鼠黑色素瘤肺转移的作用。The goal of this study was to establish the technology ofmonitoring the effects of arsenic tri- oxide on metastasis of murine malignant melanoma with in vivo bioluminescence imaging technique, and compare the superiority and disadvantage of in vivo bioluminescence imaging and conventional ani- mal experimental methods, and the anti- metastatic mechanism of arsenic trioxide was discussed. The results showed that the in vivo bioluminescence imaging could successfully be used to monitor noninva- sively and dynamically the process of the pulmonary metastasis of B16 melanoma cells and evaluate the inhibition efficacy of arsenic trioxide on the pulmonary metastasis of murine B16 melanoma cells, and compared with conventional pulmonary metastatic nodule count, the in vivo bioluminescence imaging possessed the advantages of non-invasion, real-time, dynamic visualization and hypersensitivity. Ar- senic trioxide exerted its significant anti-pulmonary metastasis effects of murine melanoma via repress- ing neovascularization of metastatic tumor to facilitate necrosis of the tumor cells.

关 键 词：活体生物发光成像技术 B16黑色素瘤 肺转移模型 三氧化二砷 血管生成 

分 类 号：R73-3[医药卫生—肿瘤]