大肠杆菌胞嘧啶脱氨基酶基因联合5-氟胞嘧啶治疗胃癌的实验研究  

A STUDY ON THE TREATMENT OF GASTRIC CANCER BY CD GENE COMBINED WITH 5-Fc IN VITRO AND IN VIVO

作　　者：郭善禹[1] 顾琴龙[2] 朱正纲[2] 尹浩然[2] 林言箴[2] 

机构地区：[1]上海第二医科大学附属第九人民医院外科,200011 [2]上海第二医科大学附属瑞金医院外科,上海消化外科研究所200025

出　　处：《肿瘤》2001年第1期11-13,共3页Tumor

基　　金：卫生部基金!(代号 98-1-312 );上海市自然基金!(代号 :98ZB14 0 2 8)资助

摘　　要：目的　观察自杀基因 CD对小鼠胃癌的体内外杀伤作用及组织病理变化。方法　应用逆转录病毒方法转导自杀基因 CD,通过体外实验观察 CD基因对小鼠胃癌 MFC细胞的杀伤性和旁观者效应 ;体内实验中首先在 6 15小鼠建立胃癌模型 ,然后分组将病毒上清注入瘤体内 ,并应用前药 (5 -氟胞嘧啶 ,5 - Fc) ,观察对肿瘤的杀伤作用及组织病理变化。结果　 CD基因在体外即显出明显的杀伤作用 ,2 0 %的转基因细胞可以杀伤 70 %～ 80 %以上的肿瘤细胞。体内实验表明 CD基因结合前药 5 - Fc可显著杀伤肿瘤 ,而单纯转导 CD基因或应用 5 - Fc并不能起到杀伤作用。结论　自杀基因 CD联合前药 5 - Fc对体内外小鼠胃癌细胞产生显著杀伤作用 ,不仅转基因细胞被杀死 。Objective To study the killing effect of suicide gene CD in treating mouse gastric cancer with transgenic assay and to observe the related clinico pathological changes. Methods CD gene was transduced with the retroviral vector. The killing effect and bystander effect of CD gene on mouse gastric cancer cell line MFC were observed. The mouse gastric cancer model was used in vivo study. The CD gene containing viral serum was injected into the tumors. The volume of the tumors in every group were measured in time. Results Significant killing effect and bystander effect were caused by CD gene in vitro,20% gene transduced cells induced 70%～80% of the total cells to death. In vivo, transduced CD gene combined with 5 Fc could cause tumor to diminish, but only induced CD gene or only 5 Fc had no killing effect. Conclusion CD/5Fc system has significant killing effect on mouse gastric cancer

关 键 词：胃肿瘤 基因治疗 CD基因 逆转录病毒 

分 类 号：R735.205[医药卫生—肿瘤]