杜仲苷对炎性环境下软骨细胞的增殖和Ⅱ型胶原蛋白分泌的影响  

The Influence of Aucubin on Proliferation of Cartilage Cells and Secretion of Collagen Type Ⅱ under Inflammatory Environment

作　　者：谢国平[1] 王胜楠[1] 姜楠[1] 

机构地区：[1]南方医科大学南方医院,广东广州510515

出　　处：《按摩与康复医学》2014年第4期213-215,共3页Chinese Manipulation and Rehabilitation Medicine

基　　金：广东省自然科学基金面上项目,编号：S2011010004415

摘　　要：目的：观察杜仲苷对IL-1β刺激大鼠体外软骨细胞的增殖及Ⅱ型胶原蛋白分泌的影响。方法：利用IL-1β刺激建立体外大鼠软骨细胞炎性模型，实验分为空白组、IL-1β组、10μM杜仲苷组、100μM杜仲苷组、500μM杜仲苷组、1000μM杜仲苷组；空白组加入10%FBS培养液，IL-1β组加入10ng/mL IL-1β＋10%FBS培养液，4个不同浓度杜仲苷组分别加入10μM、100μM、500μM、1000μM杜仲苷＋10ng/mL IL-1β＋10%FBS培养液，分别培养48小时，采用CCK8检测软骨细胞增殖活力及Ⅱ型胶原蛋白的分泌。结果：IL-1β组的OD值低于空白组（P〈0.05）；4个不同浓度杜仲苷组的OD值均高于IL-1β组（P〈0.05），且存在一定的量效关系；4个不同浓度杜仲苷组的Ⅱ型胶原蛋白表达均高于IL-1β组（P〈0.05），且存在有一定的量效关系。结论：杜仲苷可以提高体外大鼠软骨细胞炎性环境下的增殖活力，促进Ⅱ型胶原蛋白的分泌，表明杜仲苷对体外大鼠软骨细胞有一定的抗炎保护作用。Objective：To observe the influence of aucubin on the proliferation of vitro cartilage cells from IL-1βstimulated rats and their secretion of collagen type Ⅱ. Methods：Using IL-1βstimulation to establish inflammatory model of vitro cartilage cells with rats, then divided into control group, IL-1β group, 10μMaucubin group, 100μMaucubin group, 500μMaucubin group and 1000μMaucubin group; 10% FBS medium was put in control group, while 10ng/mL IL-1β＋10%FBS medium were in IL-1βgroup, and 10μM、100μM、500μM、1000μMaucubin＋10ng/mL IL-1β＋10%FBS medi-um were respectively in the four different concentration aucubin groups, all the groups were cultured for 48 hours, and used CCK8 to test prolifera-tion activity of cartilage cells and secretion of collagen type Ⅱ. Results：The OD value of IL-1βgroup was lower than that of control group （P〈0.05）;the OD value of four different concetration aucubin groups were all higher than that of IL-1βgroup （P〈0.05）, and existed a dose-response relation-ship;the expression of collagen type Ⅱ in the four different concentration aucubin groups were all higher than that of IL-1βgroup （P〈0.05）, and exist-ed a dose-response relationship. Conclusion：Aucubin can improve proliferation activity of rats＇ vitro cartilage cells under inflammatory environment, promote the secretion of collagen type Ⅱ, which indicates that aucubin has certain anti-inflammatory effect on rats＇ vitro cartilage cells.

关 键 词：杜仲苷 软骨细胞 IL-1Β 炎性环境 增殖 Ⅱ型胶原蛋白 

分 类 号：R-33[医药卫生]