癌基因P^(53)、H-ras、C-myc蛋白产物在胃癌裸鼠移植瘤中的表达及相互关系  

EXPRESSION OF P^(53),H-ras AND C-myc GENE PROTEINS IN XENOGRAFTED TUMOR IN NUDE MICE AND RELATIONSHIP AMONG THEM

作　　者：黄承良[1] 刘厚东[1] 

机构地区：[1]西藏军区总医院普外科,西藏拉萨850003

出　　处：《西南国防医药》2001年第1期10-12,共3页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的:观察癌基因p53、H—ras、C—myc蛋白产物在不同分化程度胃癌裸鼠移植瘤组织中的表达及相互关系。方法:在建立不同细胞株胃癌裸鼠移植瘤的基础上,用ABC免疫酶标法研究癌基因P53、H—ras、C—myc蛋白产物在胃癌裸鼠移植瘤中的表达。结果:癌基因P53、H—ras、C—myc在胃癌裸鼠移植瘤中的阳性表达率分别为50.00％、54.54％、63.63％。P53阳性表达与移植瘤分化程度有关.即分化越差,其阳性表达越高,P21、P62阳性表达与肿瘤分化程度无关。22例胃癌移植瘤中.有6例显示P53、P21、P62共同阳性,有10例显示P53与P21协同表达,有8例显示P53、P62协同表达,而无1例单独P53表达阳性。提示突变型p53、H—ras、C—myc异常表达与胃癌发生有密切关系。Objective: To observe the expression of P53,H-ras and C - myc gene proteins in the xenografted gastric tumor in nude mice and to study the relatioship among them. Method: Based on the establishment of different models of tumor cell strains of implanted gastric carcinoma in nude mice, the expression of P53,H -ras and C -myc gene proteins in tumor tissuses were examined with immunohistochemical method. Result: The positive rates of expression were 50. 00% for P53, 54. 54% for P21 and 63. 3% for P62, respectively. The positive expression of P53 was related to the differential level, ie. the higher the expession was, the lower the differentiation was. But the expression of P21 and P62 were not related to the differentiation. There were 6 cases that showed expression of these three gene proteins, 10 cases that showed P53 and H - ras expression and 8 cases showed P53 and C - myc expression among all 22 cases, but there was no one case that showed expression of P53 alone. Conclusion: Unusual expression of these three gene proteins has a corrlation with the gastric carcinoma.

关 键 词：胃癌 癌基因蛋白 裸鼠 表达 移植瘤 

分 类 号：R735.202[医药卫生—肿瘤]