藏药十味诃子丸的质量控制  

Quality Control of Tibetan Medicine Shiwei Hezi Pills

作　　者：张幸福 才毛 骆桂法 

机构地区：[1]青海省食品药品检验所,西宁810016

出　　处：《中国实验方剂学杂志》2014年第9期51-55,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家药品标准提高项目(民族药414)

摘　　要：目的：建立藏药十味诃子丸的质量控制方法.方法：采用显微鉴别和薄层色谱法进行定性鉴别,采用HPLC法测定羟基红花黄色素A和没食子酸的含量.结果：通过显微可鉴别出红花和诃子;TLC法可鉴别出诃子、红花、山矾叶、紫草茸、獐牙菜和藏茜草及其活性成分的特征斑点;羟基红花黄色素A回归方程为Y=2.526 3 ×10^6X-1.1957 ×10^4（r=0.999 7）线性范围0.013 6 ～0.271 7μg;没食子酸方程Y=3.052 9×10^6X-9.392×10^3（r =0.999 7）,线性范围0.157 9 ～0.947 5μg;平均回收率分别为99.9％,99.1％;RSD分别为2.2％,1.6％（n=6）.结论：建立的方法可全面控制十味诃子丸的质量,鉴别方法专属性强,定量方法准确,可有效控制该制剂的质量.Objective：To establish a method for quality control of Tibetan Medicine Shiwei Hezi Pills.Method：Traditional Tibetan medicine in prescription were identified by a series of Microstructure and TLC methods.Hydroxysafflor yellow A; gallic acid was determined by HPLC.The separation was performed on Phenomenex C18 column （4.6 mm × 250 mm,5 μm）.Result：The linear range of Hdroxysafflor yellow A and gallic acid was within the range of 0.013 6-0.271 7 μg and 0.157 9-0.947 5 μg.The average recovery was 99.9％ and 99.1,the RSD was 2.2％.and 1.6％.Conclusion：The method can be applied to the quality control of Shiwei Hezi Pills.

关 键 词：十味诃子丸 红花 诃子 紫草茸 藏茜草 獐牙菜 山矾叶 羟基红花黄色素A 没食子酸 

分 类 号：R284.1[医药卫生—中药学]