液相芯片分析技术检测乳腺浸润性导管癌患者血清中VEGF和MMP-9的临床意义  

Clinical value of VEGF and MMP-9 expression in patients with breast infiltrative ductal carcinoma using multiplexed assayes

作　　者：张君莹[1] 吴建中[2] 季明华[3] 张烨[4] 尹丽[3] 徐涛[5] 罗舟 马蓉[2] 曹海霞[2] 唐金海[5] 

机构地区：[1]徐州医学院肿瘤学教研室,江苏徐州221004 [2]江苏省肿瘤医院肿瘤中心实验室,江苏南京210009 [3]江苏省肿瘤医院放疗科 [4]江苏省肿瘤医院内科 [5]江苏省肿瘤医院普外科

出　　处：《中国肿瘤外科杂志》2014年第2期93-96,共4页Chinese Journal of Surgical Oncology

基　　金：国家自然科学基金资助项目(30840093)

摘　　要：目的利用液相芯片分析技术检测血管内皮生长因子(vascular endothelial growth factor,VEGF)、基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)在乳腺浸润性导管癌(infiltrative ductal carcinoma,IDC)、良性乳腺纤维瘤患者和正常人血清中的表达,探讨其临床诊断价值。方法采用液相芯片分析技术对301例乳腺浸润性导管癌、83例乳腺良性纤维瘤患者及40例正常人血清中VEGF、MMP-9表达水平进行检测,并分析118例IDC患者手术治疗前后VEGF和MMP-9浓度变化。结果血清中VEGF和MMP-9的表达水平:乳腺浸润性导管癌患者分别为(152.76±150.28)pg/ml和(945.09±749.43)ng/ml;乳腺纤维瘤患者为(29.86±16.10)pg/ml和(563.59±183.29)ng/ml;正常人表达量为(52.45±51.39)pg/ml和(267.33±215.60)ng/ml;IDC患者显著高于乳腺纤维瘤患者和正常人(均P<0.05)。乳腺癌患者血清中VEGF、MMP-9表达水平与年龄无关(P>0.05);而与临床分期、原发灶大小及淋巴结转移相关(均P<0.01)。118例IDC患者术后复查血清VEGF、MMP-9表达量分别(135.26±131.20)pg/ml和(680.36±551.77)ng/ml,与术前相比,表达水平明显下降(均P<0.05)。结论利用液相芯片分析技术定量分析血清中VEGF和MMP-9可成为乳腺癌临床诊断和预后评估的有效指标。Objective This study was designed to detect the levels of serum vascular endothelial growth factor（VEGF） and matrix metalloproteinase-9 （MMP-9） in patients with breast infiltrative ductal carcinoma （IDC） and to explore their clinical value. Methods A tolal of 301 cases with IDC, 83 cases of breast fibroad- enoma and 40 cases of healthy controls were recruited in this study. Levels of serum VEGF and MMP-9 were determined by Luminex multiplexed assays. Results Serum levels of VEGF and MMP-9 were respectively （ 152.76 ± 150.28） pg/ml and （945.09 ± 749.43 ） ng/ml in patients with IDC, （ 29.86 ± 16.10） pg/ml and （563.59 ± 183.29） ng/ml in breast fibroadenoma patients, （52.45 ±51.39） pg/ml and （267.33 ± 215.60） ng/ml in healthy people. The results showed that levels of serum VEGF and MMP-9 were significantly higher in IDC patients than breast fibroadenoma patients and healthy controls. However, VEGF and MMP-9 levels were correlated with lymph node metastasis, tumor size and clinical stage （ P 〈 0.01 ） ; while being independent of other clinicopathologic parameters such as age（ P 〉 0.05 ）. Moreover, a subgroup analysis showed that in pa- tients with IDC, levels of serum VEGF and MMP-9 before operation[ （ 180.89 ± 167.82） pg/ml and （903.92 ± 704.76） ng/ml ] were significantly higher than those after operation （ P 〈 0.05 ）. Conclusions The serum levels of VEGF and MMP-9 can be used as markers in diagnosis of breast infiltrative ductal carcinoma and also valuable for predicting lymph nodes metastasis.

关 键 词：乳腺浸润性导管癌 液相芯片分析技术 血管内皮生长因子 基质金属蛋白酶9 

分 类 号：R737.9[医药卫生—肿瘤]